Difference between revisions of "Microfluidics"

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= Photolithography =  
= Photolithography =  
[ https://wiki.uio.no/mn/fysikk/laglivlab/index.php/Photolithography Procedures]
[https://wiki.uio.no/mn/fysikk/laglivlab/index.php/Photolithography Procedures]
= PDMS  casting =
= PDMS  casting =

Revision as of 10:50, 3 April 2022

Designing Microfluidic masks

Some microfluidic circuit designs for a 3" wafer
  • You can use Klayout but we have more support for using Autocad.
  • Mask template for placing your designs
  • This guide for designing masks has some important tips
    • All fluid pathways have to be inside one or more closed contour(s)
    • The outer contour should be drawn in one layer (Give it a name like "Flow")
    • Any obstacles inside this outer contour must be drawn in another layer. Give it a name like "Flow inner polygons")
  • Displaying your design as PDF or otherwise is not straight forward because the resolution needed. You get a fair impression using CloudConvert which is much better than AutoCads own pdf export.
  • Example of a design where white lines (in screen shot below, black lines in PDF) are contours containing flow and green lines are inner polygons. Here is a PDF version of the file and the DWG file.
  • Rounding corners on a region
    • Command EXPLODE makes region into lines
    • Command JOIN joins lines into polylines
    • Command FILLET, downarrow to get options RADIUS and POLYLINE



PDMS casting

Assembling the chip =

Filling the chip

Water filling

Fibronectin filling

Cell filling

Running experiment