Difference between revisions of "Rotating cells"

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[[File:Electrorotation setup.jpg|thumb|Electrorotation setup. This circuit contains two D-flipflops and provides 4 output voltages that have the same signal frequency and amplitude but are phaseshifted by 90° from one to another.
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This is the main page of the rotating cells project.
 
This is the main page of the rotating cells project.
  
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-08.04. Some members of the team got started on develop the instrumentation. The prototype has been developed and checked on a breadboard. The results were not perfect and needs more work.  
 
-08.04. Some members of the team got started on develop the instrumentation. The prototype has been developed and checked on a breadboard. The results were not perfect and needs more work.  
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[[File:Students are working on the instrumentation..jpg|thumb]]
  
 
-13.04/14.04. Third online meeting within the electrorotation group. The team has decided to test out the instrumentation for electrorotation using yeast cells in order to see whether we will be able to rotate the cells. Team members have also discussed potential project ideas, where some consisted of working with cancer cells, specifically on ''tumour heterogeneity'' or researching their polarity, which would be possible to do using electrorotation. Some other ideas consisted of looking into a genetic illness called ALD, which stands for ''Adrenoleukodystrophy.'' In order to decide on the research title, the team has decided to have a meeting with a few experts in the nearby future to discuss the feasibility of these ideas.
 
-13.04/14.04. Third online meeting within the electrorotation group. The team has decided to test out the instrumentation for electrorotation using yeast cells in order to see whether we will be able to rotate the cells. Team members have also discussed potential project ideas, where some consisted of working with cancer cells, specifically on ''tumour heterogeneity'' or researching their polarity, which would be possible to do using electrorotation. Some other ideas consisted of looking into a genetic illness called ALD, which stands for ''Adrenoleukodystrophy.'' In order to decide on the research title, the team has decided to have a meeting with a few experts in the nearby future to discuss the feasibility of these ideas.

Revision as of 20:33, 14 April 2021

Electrorotation setup. This circuit contains two D-flipflops and provides 4 output voltages that have the same signal frequency and amplitude but are phaseshifted by 90° from one to another.

This is the main page of the rotating cells project.

Time line Time line link -16.03. First online meeting within the electrorotation group, in which we introduced each other and talked about our backgrounds and research interests.

-24.03. Online seminar about electrorotation and ponderomotive forces in general given by Prof. Jan Gimsa from the University of Rostock.

-29.03. Second online meeting within the electrorotation group before Easter break where we discussed our plan of action. First thing on the to-do list is to develop instrumentation that enables electrorotation.

-08.04. Some members of the team got started on develop the instrumentation. The prototype has been developed and checked on a breadboard. The results were not perfect and needs more work.

Students are working on the instrumentation..jpg

-13.04/14.04. Third online meeting within the electrorotation group. The team has decided to test out the instrumentation for electrorotation using yeast cells in order to see whether we will be able to rotate the cells. Team members have also discussed potential project ideas, where some consisted of working with cancer cells, specifically on tumour heterogeneity or researching their polarity, which would be possible to do using electrorotation. Some other ideas consisted of looking into a genetic illness called ALD, which stands for Adrenoleukodystrophy. In order to decide on the research title, the team has decided to have a meeting with a few experts in the nearby future to discuss the feasibility of these ideas.

The prototype has also been fixed - the device is now able to produce a signal with correct phase shifts using a D flip flop.

Project progression

- We need to start to develop the instrumentation that enables electrorotation. We got already the electrodes for it (see Fig. 1) and just need to add some small electrical circuit that enables the application of an alternating electrical field.

-The development of the instrumentation has been started. Members of the team followed Anders Jansen's master thesis section Method and materials, specifically Figure 3.6, in order to connect the dual D-type Flip Flop. The D flip flop used by us was different to the one used by Anders, so we had to follow the data sheet of our d flip flop in order to install it correctly, while still following Jansen's circuit diagram. D flip flop used by us was Toshiba TC74HC74AP.

Fig. 1: Electrodes for the electrorotation project that were provided by Jan Gimsa.

Literature

- Jan Gimsa "A Unified Resistor-Capacitor Model for Impedance, Dielectrophoresis, Electrorotation, and Induced Transmembrane Potential"

https://doi.org/10.1016/S0006-3495(98)77600-3

- Ørjan G. Martinsen et al. "Interface phenomena and dielectric properties of biological tissue"

https://www.mn.uio.no/fysikk/english/research/projects/bioimpedance/publications/papers/encyclop.pdf

- Master thesis Anders Jansen " A feasibility study of electrorotation as an alternative to impedance measurements"

https://www.duo.uio.no/bitstream/handle/10852/65672/1/Masteroppgave---Anders-Jansen.pdf