Difference between revisions of "Unresolved issues"

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* The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber.
 
* The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber.
 
* Adding soft surfacant to make sure the bubbles detach from the surface.
 
* Adding soft surfacant to make sure the bubbles detach from the surface.
 +
* Being highly aware of pressure levels when adding liquids to the chambers. Even the smallest changes in pressure can result in air bubbles.
 
* [https://darwin-microfluidics.com/products/microfluidic-bubble-trap Bubble Trap for Microfluidics Kit] provided by [https://www.elveflow.com/ Elveflow] which gets rid of the air bubbles before they enter the chip.
 
* [https://darwin-microfluidics.com/products/microfluidic-bubble-trap Bubble Trap for Microfluidics Kit] provided by [https://www.elveflow.com/ Elveflow] which gets rid of the air bubbles before they enter the chip.
 
*[https://www.elveflow.com/microfluidic-reviews/general-microfluidics/air-bubbles-and-microfluidics/#:~:text=Bubbles%20dissolution%3A%20For%20those%20that,to%20dissolve%20into%20the%20liquid. Air bubbles and microfluidics: Tips and tricks to remove them]
 
*[https://www.elveflow.com/microfluidic-reviews/general-microfluidics/air-bubbles-and-microfluidics/#:~:text=Bubbles%20dissolution%3A%20For%20those%20that,to%20dissolve%20into%20the%20liquid. Air bubbles and microfluidics: Tips and tricks to remove them]

Revision as of 20:16, 31 July 2022

There are several unresolved issues present when it comes to the experiment part of the project.

The following issues are:

Keeping the cells alive inside the chip

Controlling the CO2 levels

Air bubbles

When filling chambers with liquid, the bubbles result in the cells dying prematurely

Possible solutions to air bubbles

  • The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber.
  • Adding soft surfacant to make sure the bubbles detach from the surface.
  • Being highly aware of pressure levels when adding liquids to the chambers. Even the smallest changes in pressure can result in air bubbles.
  • Bubble Trap for Microfluidics Kit provided by Elveflow which gets rid of the air bubbles before they enter the chip.
  • Air bubbles and microfluidics: Tips and tricks to remove them