Unresolved issues

There are several unresolved issues present when it comes to the experiment part of the project.

The following issues are:

Keeping the cells alive inside the chip

In order to achieve useful results

Possible solutions:

Controlling the CO2 levels

Environment

Possible solutions:

Air bubbles

When filling chambers with liquid, the bubbles can result in the cells dying prematurely and/or inhibiting the flow.

Possible solutions to air bubbles:

• The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber.
• Adding soft surfacant to make sure the bubbles detach from the surface.
• Being highly aware of pressure levels when adding liquids to the chambers. Even the smallest changes in pressure can result in air bubbles.
• Bubble Trap for Microfluidics Kit provided by Elveflow which gets rid of the air bubbles before they enter the chip.
• Air bubbles and microfluidics: Tips and tricks to remove them
• Methods & techniques for perfusion cell culture