Difference between revisions of "WC"

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(Created page with " == Description == The WC (Wright Chu) medium is a commonly used fully-defined medium for culturing of limnic algae. It was first described by Guillard & Lorenzen, and the mos...")
 
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== Description ==
 
== Description ==
−
The WC (Wright Chu) medium is a commonly used fully-defined medium for culturing of limnic algae. It was first described by Guillard & Lorenzen, and the most-commonly used recipe includes modifications as reported by Makulla.
+
The WC ('''W'''right '''C'''hu) medium is a commonly used fully-defined medium for culturing of limnic algae. It was first described by Guillard & Lorenzen<ref>Guillard, R.R.L. and Lorenzen, C.J. (1972) '''Yellow-green algae with chlorophyllide ''c'''''. ''Journal of Phycology'', 8:1, 10–18. DOI: </ref>, and the most-commonly used recipe includes modifications as reported by Makulla<ref>Makulla, A. (2000) '''Fatty acid composition of ''Scenedesmus obliquus'': Correlation to dilution rates'''. ''Limnologica'', 30:2, 162–168. DOI:</ref>.
 +
== Recipe ==
  
−
 
+
Dissolve TES buffer in Type I purified water (e.g., Milli-Q) using a magnetic stirrer and add 1 mL/L of stock solutions and trace metals in numerical order ('''1–7'''). Autoclave appropriately and add 1 mL/L vitamins ('''8''') through a sterile 0.22 μm polystyrene (PES) filter once cooled down to room temperature.
−
== Recipe ==
+
{| class="wikitable"
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{| class="MsoTableGrid"
 +
 +
  | colspan="2" |
 +
<nowiki>  </nowiki>'''Buffer'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>[M] g/mol
 +
 
 +
  |
 +
<nowiki>  </nowiki>mg/5 L
 +
 
 +
  |
 +
<nowiki>  </nowiki>mg/L
 +
 
 +
  |   
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>TES
 +
 
 +
  |
 +
<nowiki>  </nowiki>229.25
 +
 
 +
  |
 +
<nowiki>  </nowiki>575
 +
 
 +
  |
 +
<nowiki>  </nowiki>115
 +
 
 +
  |   
 +
 +
|-
 +
  | 
 +
 
 +
  | 
 +
 
 +
  | 
 +
 
 +
  | 
 +
 
 +
  | 
 +
 
 +
  |   
 +
 +
|-
 +
  | colspan="2" |
 +
<nowiki>  </nowiki>'''Stock
 +
<nowiki> </nowiki> solutions'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>[M] g/mol
 +
 
 +
  |
 +
<nowiki>  </nowiki>g/L
 +
 
 +
  |
 +
<nowiki>  </nowiki>g/500 mL
 +
 
 +
  |
 +
<nowiki>  </nowiki>g/250 mL 
 +
 +
|-
 +
  |
 +
<nowiki>  </nowiki>'''1'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>CaCl<sub>2</sub> · 2 H<sub>2</sub>O
 +
 
 +
  |
 +
<nowiki>  </nowiki>147.02
 +
 
 +
  |
 +
<nowiki>  </nowiki>36.8
 +
 
 +
  |
 +
<nowiki>  </nowiki>18.4
 +
 
 +
  |
 +
<nowiki>  </nowiki>9.20 
 +
 +
|-
 +
  |
 +
<nowiki>  </nowiki>'''2'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>MgSO<sub>4</sub> · 7 H<sub>2</sub>O
 +
 
 +
  |
 +
<nowiki>  </nowiki>246.48
 +
 
 +
  |
 +
<nowiki>  </nowiki>37.0
 +
 
 +
  |
 +
<nowiki>  </nowiki>18.5
 +
 
 +
  |
 +
<nowiki>  </nowiki>9.25 
 +
 +
|-
 +
  |
 +
<nowiki>  </nowiki>'''3'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>NaHCO<sub>3</sub>
 +
 
 +
  |
 +
<nowiki>  </nowiki>84.01
 +
 
 +
  |
 +
<nowiki>  </nowiki>12.6
 +
 
 +
  |
 +
<nowiki>  </nowiki>6.3
 +
 
 +
  |
 +
<nowiki>  </nowiki>3.15 
 +
 +
|-
 +
  |
 +
<nowiki>  </nowiki>'''4'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>K<sub>2</sub>HPO<sub>4</sub>
 +
 
 +
  |
 +
<nowiki>  </nowiki>174.18
 +
 
 +
  |
 +
<nowiki>  </nowiki>8.7
 +
 
 +
  |
 +
<nowiki>  </nowiki>4.4
 +
 
 +
  |
 +
<nowiki>  </nowiki>2.18 
 +
 +
|-
 +
  |
 +
<nowiki>  </nowiki>'''5'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>NaNO<sub>3</sub>
 +
 
 +
  |
 +
<nowiki>  </nowiki>85.01
 +
 
 +
  |
 +
<nowiki>  </nowiki>85.0
 +
 
 +
  |
 +
<nowiki>  </nowiki>42.5
 +
 
 +
  |
 +
<nowiki>  </nowiki>21.25 
 +
 +
|-
 +
  |
 +
<nowiki>  </nowiki>'''6'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>NaSiO<sub>3</sub> · 5 H<sub>2</sub>O
 +
 
 +
  |
 +
<nowiki>  </nowiki>212.16
 +
 
 +
  |
 +
<nowiki>  </nowiki>21.2
 +
 
 +
  |
 +
<nowiki>  </nowiki>10.6
 +
 
 +
  |
 +
<nowiki>  </nowiki>5.30 
 +
 +
|-
 +
  |
 +
<nowiki>  </nowiki>''' '''
 +
 
 +
  | 
 +
 
 +
  | 
 +
 
 +
  | 
 +
 
 +
  | 
 +
 
 +
  |   
 +
 +
|-
 +
  |
 +
<nowiki>  </nowiki>'''7'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>'''Trace metals'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>[M] g/mol
 +
 
 +
  |
 +
<nowiki>  </nowiki>mg/L
 +
 
 +
  |
 +
<nowiki>  </nowiki>mg/500 mL
 +
 
 +
  |
 +
<nowiki>  </nowiki>mg/250 mL 
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>Na<sub>2</sub> · EDTA
 +
 
 +
  |
 +
<nowiki>  </nowiki>372.24
 +
 
 +
  |
 +
<nowiki>  </nowiki>4360
 +
 
 +
  |
 +
<nowiki>  </nowiki>2 180
 +
 
 +
  |
 +
<nowiki>  </nowiki>1 090.0 
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>FeCl<sub>3</sub> · 6 H<sub>2</sub>O
 +
 
 +
  |
 +
<nowiki>  </nowiki>270.32
 +
 
 +
  |
 +
<nowiki>  </nowiki>315
 +
 
 +
  |
 +
<nowiki>  </nowiki>1 575
 +
 
 +
  |
 +
<nowiki>  </nowiki>787.5 
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>CuSO<sub>4</sub> · 5 H<sub>2</sub>O
 +
 
 +
  |
 +
<nowiki>  </nowiki>249.69
 +
 
 +
  |
 +
<nowiki>  </nowiki>10
 +
 
 +
  |
 +
<nowiki>  </nowiki>5
 +
 
 +
  |
 +
<nowiki>  </nowiki>2.5 
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>ZnSO<sub>4</sub> · 7 H<sub>2</sub>O
 +
 
 +
  |
 +
<nowiki>  </nowiki>287.54
 +
 
 +
  |
 +
<nowiki>  </nowiki>22
 +
 
 +
  |
 +
<nowiki>  </nowiki>11
 +
 
 +
  |
 +
<nowiki>  </nowiki>5.5 
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>CoCl<sub>2</sub> · 6 H<sub>2</sub>O
 +
 
 +
  |
 +
<nowiki>  </nowiki>237.93
 +
 
 +
  |
 +
<nowiki>  </nowiki>10
 +
 
 +
  |
 +
<nowiki>  </nowiki>5
 +
 
 +
  |
 +
<nowiki>  </nowiki>2.5 
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>MnCl<sub>2</sub> · 4 H2O
 +
 
 +
  |
 +
<nowiki>  </nowiki>197.90
 +
 
 +
  |
 +
<nowiki>  </nowiki>180
 +
 
 +
  |
 +
<nowiki>  </nowiki>90
 +
 
 +
  |
 +
<nowiki>  </nowiki>45 
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>NaMoO<sub>4</sub> · 2 H<sub>2</sub>O
 +
 
 +
  |
 +
<nowiki>  </nowiki>241.95
 +
 
 +
  |
 +
<nowiki>  </nowiki>6
 +
 
 +
  |
 +
<nowiki>  </nowiki>3
 +
 
 +
  |
 +
<nowiki>  </nowiki>1.5 
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>H<sub>3</sub>BO<sub>3</sub>
 +
 
 +
  |
 +
<nowiki>  </nowiki>61.83
 +
 
 +
  |
 +
<nowiki>  </nowiki>1000
 +
 
 +
  |
 +
<nowiki>  </nowiki>500
 +
 
 +
  |
 +
<nowiki>  </nowiki>250.0 
 +
 +
|-
 +
  | 
 +
 
 +
  | 
 +
 
 +
  | 
 +
 
 +
  | 
 +
 
 +
  | 
 +
 
 +
  |   
 +
 +
|-
 +
  |
 +
<nowiki>  </nowiki>'''8'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>'''Vitamins'''
 +
 
 +
  |
 +
<nowiki>  </nowiki>[M] g/mol
 +
 
 +
  |
 +
<nowiki>  </nowiki>mg/L
 +
 
 +
  |
 +
<nowiki>  </nowiki>mg/500 mL
 +
 
 +
  |
 +
<nowiki>  </nowiki>mg/250 mL 
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>Thiamin · HCl
 +
 
 +
  |
 +
<nowiki>  </nowiki>337.30
 +
 
 +
  |
 +
<nowiki>  </nowiki>100.0
 +
 
 +
  |
 +
<nowiki>  </nowiki>50.00
 +
 
 +
  |
 +
<nowiki>  </nowiki>25.000 
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>Biotin
 +
 
 +
  |
 +
<nowiki>  </nowiki>244.31
 +
 
 +
  |
 +
<nowiki>  </nowiki>0.5
 +
 
 +
  |
 +
<nowiki>  </nowiki>0.25
 +
 
 +
  |
 +
<nowiki>  </nowiki>0.125 
 +
 +
|-
 +
  | 
 +
 
 +
  |
 +
<nowiki>  </nowiki>B<sub>12</sub>
 +
 
 +
  |
 +
<nowiki>  </nowiki>1355.38
 +
 
 +
  |
 +
<nowiki>  </nowiki>0.5
 +
 
 +
  |
 +
<nowiki>  </nowiki>0.25
 +
 
 +
  |
 +
<nowiki>  </nowiki>0.125 
 +
 +
|}
  
  
  
 
== References ==
 
== References ==

Revision as of 17:11, 18 October 2016

Description

The WC (Wright Chu) medium is a commonly used fully-defined medium for culturing of limnic algae. It was first described by Guillard & Lorenzen[1], and the most-commonly used recipe includes modifications as reported by Makulla[2].

Recipe

Dissolve TES buffer in Type I purified water (e.g., Milli-Q) using a magnetic stirrer and add 1 mL/L of stock solutions and trace metals in numerical order (1–7). Autoclave appropriately and add 1 mL/L vitamins (8) through a sterile 0.22 μm polystyrene (PES) filter once cooled down to room temperature.

Buffer

[M] g/mol

mg/5 L

mg/L

TES

229.25

575

115

Stock solutions

[M] g/mol

g/L

g/500 mL

g/250 mL

1

CaCl2 · 2 H2O

147.02

36.8

18.4

9.20

2

MgSO4 · 7 H2O

246.48

37.0

18.5

9.25

3

NaHCO3

84.01

12.6

6.3

3.15

4

K2HPO4

174.18

8.7

4.4

2.18

5

NaNO3

85.01

85.0

42.5

21.25

6

NaSiO3 · 5 H2O

212.16

21.2

10.6

5.30

 

7

Trace metals

[M] g/mol

mg/L

mg/500 mL

mg/250 mL

Na2 · EDTA

372.24

4360

2 180

1 090.0

FeCl3 · 6 H2O

270.32

315

1 575

787.5

CuSO4 · 5 H2O

249.69

10

5

2.5

ZnSO4 · 7 H2O

287.54

22

11

5.5

CoCl2 · 6 H2O

237.93

10

5

2.5

MnCl2 · 4 H2O

197.90

180

90

45

NaMoO4 · 2 H2O

241.95

6

3

1.5

H3BO3

61.83

1000

500

250.0

8

Vitamins

[M] g/mol

mg/L

mg/500 mL

mg/250 mL

Thiamin · HCl

337.30

100.0

50.00

25.000

Biotin

244.31

0.5

0.25

0.125

B12

1355.38

0.5

0.25

0.125


References

  1. ↑ Guillard, R.R.L. and Lorenzen, C.J. (1972) Yellow-green algae with chlorophyllide c. Journal of Phycology, 8:1, 10–18. DOI:
  2. ↑ Makulla, A. (2000) Fatty acid composition of Scenedesmus obliquus: Correlation to dilution rates. Limnologica, 30:2, 162–168. DOI:
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