Difference between revisions of "WC"
From mn.ibv.aqua
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Revision as of 17:35, 18 October 2016
Description
The WC (Wright Chu) medium is a commonly used fully-defined medium for culturing of limnic algae. It was first described by Guillard & Lorenzen[1], and the most-commonly used recipe includes modifications as reported by Makulla[2].
Recipe
Dissolve TES buffer in Type I purified water (e.g., Milli-Q) using a magnetic stirrer and add 1 mL/L of stock solutions and trace metals in numerical order (1–7). Autoclave appropriately and add 1 mL/L vitamins (8) through a sterile 0.22 μm polystyrene (PES) filter once cooled down to room temperature.
| Buffer | |
|---|---|
| TES | |
| [M] g/mol | 229.25 |
| mg/5 L | 575 |
| mg/L | 115 |
| Stock solution | 1 | 2 | 3 | 4 | 5 | 6 |
|---|---|---|---|---|---|---|
| CaCl2 · 2 H2O | MgSO4 · 7 H2O | NaHCO3 | K2HPO4 | NaNO3 | NaSiO3 · 5 H2O | |
| [M] g/mol | ||||||
| g/L | ||||||
| g/500 mL | ||||||
| g/250 mL |
| Stock solution | 7: Trace metals | |||||||
|---|---|---|---|---|---|---|---|---|
| Na2 · EDTA | FeCl3 · 6 H2O | CuSO4 · 5 H2O | ZnSO4 · 7 H2O | CoCl2 · 6 H2O | MnCl2 · 4 H2O | NaMoO4 · 2 H2O | H3BO3 | |
| [M] g/mol | ||||||||
| g/L | ||||||||
| g/500 mL | ||||||||
| g/250 mL |
| Stock solution | 8: Vitamins | ||
|---|---|---|---|
| Thiamin · HCl | Biotin | B12 | |
| [M] g/mol | |||
| g/L | |||
| g/500 mL | |||
| g/250 mL |
