Difference between revisions of "WC"
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| + | !Buffer | ||
| + | !Stock solution 1 | ||
| + | !Stock solution 2 | ||
| + | !3 | ||
| + | !4 | ||
| + | !5 | ||
| + | !6 | ||
| + | !7: Trace metals | ||
! | ! | ||
! | ! | ||
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| + | ! | ||
| + | ! | ||
| + | ! | ||
| + | ! | ||
| + | !8: Vitamins | ||
| + | ! | ||
| + | ! | ||
| + | |- | ||
| + | | | ||
| + | |TES | ||
| + | |CaCl<sub>2</sub> · 2 H<sub>2</sub>O | ||
| + | |MgSO<sub>4</sub> · 7 H<sub>2</sub>O | ||
| + | |NaHCO<sub>3</sub> | ||
| + | |K<sub>2</sub>HPO<sub>4</sub> | ||
| + | |NaNO<sub>3</sub> | ||
| + | | NaSiO<sub>3</sub> · 5 H<sub>2</sub>O | ||
| + | |Na<sub>2</sub> · EDTA | ||
| + | |FeCl<sub>3</sub> · 6 H<sub>2</sub>O | ||
| + | |CuSO<sub>4</sub> · 5 H<sub>2</sub>O | ||
| + | |ZnSO<sub>4</sub> · 7 H<sub>2</sub>O | ||
| + | |CoCl<sub>2</sub> · 6 H<sub>2</sub>O | ||
| + | |MnCl<sub>2</sub> · 4 H<sub>2</sub>O | ||
| + | |NaMoO<sub>4</sub> · 2 H<sub>2</sub>O | ||
| + | |H<sub>3</sub>BO<sub>3</sub> | ||
| + | |Thiamin · HCl | ||
| + | |Biotin | ||
| + | |B<sub>12</sub> | ||
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Revision as of 17:16, 18 October 2016
Description
The WC (Wright Chu) medium is a commonly used fully-defined medium for culturing of limnic algae. It was first described by Guillard & Lorenzen[1], and the most-commonly used recipe includes modifications as reported by Makulla[2].
Recipe
Dissolve TES buffer in Type I purified water (e.g., Milli-Q) using a magnetic stirrer and add 1 mL/L of stock solutions and trace metals in numerical order (1–7). Autoclave appropriately and add 1 mL/L vitamins (8) through a sterile 0.22 μm polystyrene (PES) filter once cooled down to room temperature.
| Buffer | Stock solution 1 | Stock solution 2 | 3 | 4 | 5 | 6 | 7: Trace metals | 8: Vitamins | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TES | CaCl2 · 2 H2O | MgSO4 · 7 H2O | NaHCO3 | K2HPO4 | NaNO3 | NaSiO3 · 5 H2O | Na2 · EDTA | FeCl3 · 6 H2O | CuSO4 · 5 H2O | ZnSO4 · 7 H2O | CoCl2 · 6 H2O | MnCl2 · 4 H2O | NaMoO4 · 2 H2O | H3BO3 | Thiamin · HCl | Biotin | B12 | |
|
Buffer |
[M] g/mol |
mg/5 L |
mg/L |
||
|
TES |
229.25 |
575 |
115 |
||
|
Stock solutions |
[M] g/mol |
g/L |
g/500 mL |
g/250 mL | |
|
1 |
CaCl2 · 2 H2O |
147.02 |
36.8 |
18.4 |
9.20 |
|
2 |
MgSO4 · 7 H2O |
246.48 |
37.0 |
18.5 |
9.25 |
|
3 |
NaHCO3 |
84.01 |
12.6 |
6.3 |
3.15 |
|
4 |
K2HPO4 |
174.18 |
8.7 |
4.4 |
2.18 |
|
5 |
NaNO3 |
85.01 |
85.0 |
42.5 |
21.25 |
|
6 |
NaSiO3 · 5 H2O |
212.16 |
21.2 |
10.6 |
5.30 |
|
|
|||||
|
7 |
Trace metals |
[M] g/mol |
mg/L |
mg/500 mL |
mg/250 mL |
|
Na2 · EDTA |
372.24 |
4360 |
2 180 |
1 090.0 | |
|
FeCl3 · 6 H2O |
270.32 |
315 |
1 575 |
787.5 | |
|
CuSO4 · 5 H2O |
249.69 |
10 |
5 |
2.5 | |
|
ZnSO4 · 7 H2O |
287.54 |
22 |
11 |
5.5 | |
|
CoCl2 · 6 H2O |
237.93 |
10 |
5 |
2.5 | |
|
MnCl2 · 4 H2O |
197.90 |
180 |
90 |
45 | |
|
NaMoO4 · 2 H2O |
241.95 |
6 |
3 |
1.5 | |
|
H3BO3 |
61.83 |
1000 |
500 |
250.0 | |
|
8 |
Vitamins |
[M] g/mol |
mg/L |
mg/500 mL |
mg/250 mL |
|
Thiamin · HCl |
337.30 |
100.0 |
50.00 |
25.000 | |
|
Biotin |
244.31 |
0.5 |
0.25 |
0.125 | |
|
B12 |
1355.38 |
0.5 |
0.25 |
0.125 | |
