WC
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Revision as of 17:33, 18 October 2016 by Raoulw@uio.no (talk | contribs)
Description
The WC (Wright Chu) medium is a commonly used fully-defined medium for culturing of limnic algae. It was first described by Guillard & Lorenzen[1], and the most-commonly used recipe includes modifications as reported by Makulla[2].
Recipe
Dissolve TES buffer in Type I purified water (e.g., Milli-Q) using a magnetic stirrer and add 1 mL/L of stock solutions and trace metals in numerical order (1–7). Autoclave appropriately and add 1 mL/L vitamins (8) through a sterile 0.22 μm polystyrene (PES) filter once cooled down to room temperature.
Buffer | |
---|---|
TES | |
[M] g/mol | 229.25 |
mg/5 L | 575 |
mg/L | 115 |
Stock solution 1 | Stock solution 2 | Stock solution 3 | Stock solution 4 | Stock solution 5 | Stock solution 6 | |
---|---|---|---|---|---|---|
CaCl2 · 2 H2O | MgSO4 · 7 H2O | NaHCO3 | K2HPO4 | NaNO3 | NaSiO3 · 5 H2O | |
[M] g/mol | ||||||
g/L | ||||||
g/500 mL | ||||||
g/250 mL |
Stock solution 7: Trace metals | ||||||||
---|---|---|---|---|---|---|---|---|
Na2 · EDTA | FeCl3 · 6 H2O | CuSO4 · 5 H2O | ZnSO4 · 7 H2O | CoCl2 · 6 H2O | MnCl2 · 4 H2O | NaMoO4 · 2 H2O | H3BO3 | |
[M] g/mol | ||||||||
g/L | ||||||||
g/500 mL | ||||||||
g/250 mL |
Stock solution 8: Vitamins | |||
---|---|---|---|
Thiamin · HCl | Biotin | B12 | |
[M] g/mol | |||
g/L | |||
g/500 mL | |||
g/250 mL |