ADaM
Description
ADaM is short for "Aachener Daphnien-Medium" and is a easy to prepare, well-established medium for a variety of Daphnia and other limnic zooplankton species species. The following recipe is based on the original publication by Klüttgen et al.[1], with a modified selenium content first mentioned in Ebert et al.[2].
Recipe
To prepare ADaM, a high quality sea salt for scientific purposes is needed. Specifically, two sea salts have been frequently used in laboratories all over the world to prepare ADaM (This section will be updated soon to accommodate purchase links):
- Crystal Sea® Bioassay Laboratory Formula (Marine Enterprises International; Baltimore, MD, USA)
- hw-Marinemix® professional (Wiegandt; Krefeld, Germany)
To complete the medium, three stock solutions are needed. They should be prepared in quartz glass bottles using Type I purified water (e.g. Milli-Q water) and autoclaved after visible solution of the compounds.
| Stock solution | Compound | g/L |
|---|---|---|
| A | CaCl2 • H2O | 117.60 |
| B | NaHCO3 | 25.20 |
| C | SeO2 | 0.07 |
The medium itself is prepared by first dissolving sea salt in distilled water and then adding stock solutions A, B, and C in order.
| Final volume (L) | Sea salt (g) | Stock solution A (mL) | Stock solution B (mL) | Stock solution C (mL) |
|---|---|---|---|---|
| 10 | 3.33 | 23 | 22 | 1 |
| 50 | 16.60 | 115 | 110 | 5 |
| 60 | 19.90 | 138 | 132 | 6 |
The finished medium should aerated through a 0.22 μm polystyrene filter for at least a day before use.
References
- ↑ Klüttgen, B., Dülmer, U., Engels, M. & Ratte, H.T. (1994) ADaM, an artificial freshwater for the culture of zooplankton. Water Research, 28:3, 743–746.
- ↑ Ebert, D., Zschokke-Rohringer, C.D. & Carius, H.J. (1998) Within- and between-population variation for resistance of Daphnia magna to the bacterial endoparasite Pasteuria ramosa. Proceedings of the Royal Society B, 265:1410, 2127–2134.
