WC

Description

The WC (Wright Chu) medium is a commonly used fully-defined medium for culturing of limnic algae. It was first described by Guillard & Lorenzen, and the most-commonly used recipe includes modifications as reported by Makulla.

Recipe

Dissolve TES buffer in Type I purified water (e.g., Milli-Q) using a magnetic stirrer and add 1 mL/L of stock solutions and trace metals in numerical order (1–7). Autoclave appropriately and add 1 mL/L vitamins (8) through a sterile 0.22 μm polystyrene (PES) filter once cooled down to room temperature.

	Buffer
	TES
[M] g/mol	229.25
mg/5 L	575
mg/L	115

	Stock solution 1	Stock solution 2	Stock solution 3	Stock solution 4	Stock solution 5	Stock solution 6
	CaCl2 · 2 H2O	MgSO4 · 7 H2O	NaHCO3	K2HPO4	NaNO3	NaSiO3 · 5 H2O
[M] g/mol
g/L
g/500 mL
g/250 mL

	Stock solution 7: Trace metals
	Na2 · EDTA	FeCl3 · 6 H2O	CuSO4 · 5 H2O	ZnSO4 · 7 H2O	CoCl2 · 6 H2O	MnCl2 · 4 H2O	NaMoO4 · 2 H2O	H3BO3
[M] g/mol
g/L
g/500 mL
g/250 mL

	Stock solution 8: Vitamins
	Thiamin · HCl	Biotin	B12
[M] g/mol
g/L
g/500 mL
g/250 mL

Buffer		[M] g/mol	mg/5 L	mg/L
	TES	229.25	575	115
Stock solutions		[M] g/mol	g/L	g/500 mL	g/250 mL
1	CaCl2 · 2 H2O	147.02	36.8	18.4	9.20
2	MgSO4 · 7 H2O	246.48	37.0	18.5	9.25
3	NaHCO3	84.01	12.6	6.3	3.15
4	K2HPO4	174.18	8.7	4.4	2.18
5	NaNO3	85.01	85.0	42.5	21.25
6	NaSiO3 · 5 H2O	212.16	21.2	10.6	5.30
7	Trace metals	[M] g/mol	mg/L	mg/500 mL	mg/250 mL
	Na2 · EDTA	372.24	4360	2 180	1 090.0
	FeCl3 · 6 H2O	270.32	315	1 575	787.5
	CuSO4 · 5 H2O	249.69	10	5	2.5
	ZnSO4 · 7 H2O	287.54	22	11	5.5
	CoCl2 · 6 H2O	237.93	10	5	2.5
	MnCl2 · 4 H2O	197.90	180	90	45
	NaMoO4 · 2 H2O	241.95	6	3	1.5
	H3BO3	61.83	1000	500	250.0
8	Vitamins	[M] g/mol	mg/L	mg/500 mL	mg/250 mL
	Thiamin · HCl	337.30	100.0	50.00	25.000
	Biotin	244.31	0.5	0.25	0.125
	B12	1355.38	0.5	0.25	0.125