Difference between revisions of "ADaM"
From mn.ibv.aqua
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== Description == | == Description == | ||
| − | ADaM is short for "'''A'''achener '''Da'''phnien-'''M'''edium" and is a easy to prepare, well-established medium for a variety of ''Daphnia'' and other limnic zooplankton species species. The following recipe is based on the original publication by Klüttgen et al.<ref> | + | ADaM is short for "'''A'''achener '''Da'''phnien-'''M'''edium" and is a easy to prepare, well-established medium for a variety of ''Daphnia'' and other limnic zooplankton species species. The following recipe is based on the original publication by Klüttgen et al.<ref>Klüttgen, B., Dülmer, U., Engels, M. & Ratte, H.T. (1994) '''ADaM, an artificial freshwater for the culture of zooplankton'''. ''Water Research'', 28:3, 743–746. |
| − | </ref>, with a modified selenium content first mentioned in Ebert et al.<ref> | + | </ref>, with a modified selenium content first mentioned in Ebert et al.<ref>Ebert, D., Zschokke-Rohringer, C.D. & Carius, H.J. (1998) '''Within- and between-population variation for resistance of ''Daphnia magna ''to the bacterial endoparasite ''Pasteuria ramosa''.''' ''Proceedings of the Royal Society B'', 265:1410, 2127–2134.</ref>. |
== Recipe == | == Recipe == | ||
| − | To prepare ADaM, a high quality sea salt for scientific purposes is needed. Specifically, two sea salts have been frequently used in laboratories all over the world to prepare ADaM: | + | To prepare ADaM, a high quality sea salt for scientific purposes is needed. Specifically, two sea salts have been frequently used in laboratories all over the world to prepare ADaM (This section will be updated soon to accommodate purchase links): |
* Crystal Sea<sup>®</sup> Bioassay Laboratory Formula (Marine Enterprises International; Baltimore, MD, USA) | * Crystal Sea<sup>®</sup> Bioassay Laboratory Formula (Marine Enterprises International; Baltimore, MD, USA) | ||
* hw-Marinemix<sup>®</sup> professional (Wiegandt; Krefeld, Germany) | * hw-Marinemix<sup>®</sup> professional (Wiegandt; Krefeld, Germany) | ||
| − | + | ||
| + | Furthermore, three stock solutions are needed: | ||
| + | {| class="wikitable" | ||
| + | !Stock solution | ||
| + | !Compound | ||
| + | !g/L | ||
| + | |- | ||
| + | |A | ||
| + | |CaCl<sub>2</sub> • H<sub>2</sub>O | ||
| + | |117.60 | ||
| + | |- | ||
| + | |B | ||
| + | |NaHCO<sub>3</sub> | ||
| + | |25.20 | ||
| + | |- | ||
| + | |C | ||
| + | |SeO<sub>2</sub> | ||
| + | |0.07 | ||
| + | |} | ||
| + | |||
| + | == References == | ||
Revision as of 16:27, 17 October 2016
Description
ADaM is short for "Aachener Daphnien-Medium" and is a easy to prepare, well-established medium for a variety of Daphnia and other limnic zooplankton species species. The following recipe is based on the original publication by Klüttgen et al.[1], with a modified selenium content first mentioned in Ebert et al.[2].
Recipe
To prepare ADaM, a high quality sea salt for scientific purposes is needed. Specifically, two sea salts have been frequently used in laboratories all over the world to prepare ADaM (This section will be updated soon to accommodate purchase links):
- Crystal Sea® Bioassay Laboratory Formula (Marine Enterprises International; Baltimore, MD, USA)
- hw-Marinemix® professional (Wiegandt; Krefeld, Germany)
Furthermore, three stock solutions are needed:
| Stock solution | Compound | g/L |
|---|---|---|
| A | CaCl2 • H2O | 117.60 |
| B | NaHCO3 | 25.20 |
| C | SeO2 | 0.07 |
References
- ↑ Klüttgen, B., Dülmer, U., Engels, M. & Ratte, H.T. (1994) ADaM, an artificial freshwater for the culture of zooplankton. Water Research, 28:3, 743–746.
- ↑ Ebert, D., Zschokke-Rohringer, C.D. & Carius, H.J. (1998) Within- and between-population variation for resistance of Daphnia magna to the bacterial endoparasite Pasteuria ramosa. Proceedings of the Royal Society B, 265:1410, 2127–2134.
