ADaM

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Description

ADaM is short for "Aachener Daphnien-Medium" and is a easy to prepare, well-established medium for a variety of Daphnia and other limnic zooplankton species species. The following recipe is based on the original publication by Klüttgen et al.[1], with a modified selenium content first mentioned in Ebert et al.[2].

Recipe

To prepare ADaM, a high quality sea salt for scientific purposes is needed. Specifically, two sea salts have been frequently used in laboratories all over the world to prepare ADaM (This section will be updated soon to accommodate purchase links):

  • Crystal Sea® Bioassay Laboratory Formula (Marine Enterprises International; Baltimore, MD, USA)
  • hw-Marinemix® professional (Wiegandt; Krefeld, Germany)

Furthermore, three stock solutions are needed:

Stock solution Compound g/L
A CaCl2 • H2O 117.60
B NaHCO3 25.20
C SeO2 0.07

References

  1. Klüttgen, B., Dülmer, U., Engels, M. & Ratte, H.T. (1994) ADaM, an artificial freshwater for the culture of zooplankton. Water Research, 28:3, 743–746.
  2. Ebert, D., Zschokke-Rohringer, C.D. & Carius, H.J. (1998) Within- and between-population variation for resistance of Daphnia magna to the bacterial endoparasite Pasteuria ramosa. Proceedings of the Royal Society B, 265:1410, 2127–2134.