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| − | <nowiki> </nowiki>'''Buffer'''
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| − | <nowiki> </nowiki>[M] g/mol
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| − | <nowiki> </nowiki>229.25
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| − | <nowiki> </nowiki>575
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| − | <nowiki> </nowiki> solutions'''
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| − | <nowiki> </nowiki>[M] g/mol
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| − | <nowiki> </nowiki>g/L
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| − | <nowiki> </nowiki>g/500 mL
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| − | <nowiki> </nowiki>g/250 mL
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| − | <nowiki> </nowiki>'''1'''
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| − |
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| − | <nowiki> </nowiki>CaCl<sub>2</sub> · 2 H<sub>2</sub>O
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| − | <nowiki> </nowiki>147.02
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| − | <nowiki> </nowiki>36.8
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| − | <nowiki> </nowiki>18.4
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| − | <nowiki> </nowiki>9.20
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| − | <nowiki> </nowiki>'''2'''
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| − | <nowiki> </nowiki>MgSO<sub>4</sub> · 7 H<sub>2</sub>O
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| − | <nowiki> </nowiki>246.48
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| − | <nowiki> </nowiki>37.0
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| − | <nowiki> </nowiki>18.5
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| − | <nowiki> </nowiki>9.25
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| − | <nowiki> </nowiki>'''3'''
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| − | <nowiki> </nowiki>NaHCO<sub>3</sub>
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| − | <nowiki> </nowiki>84.01
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| − | <nowiki> </nowiki>12.6
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| − | <nowiki> </nowiki>6.3
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| − | <nowiki> </nowiki>3.15
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| − | <nowiki> </nowiki>'''4'''
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| − | <nowiki> </nowiki>K<sub>2</sub>HPO<sub>4</sub>
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| − | <nowiki> </nowiki>174.18
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| − | <nowiki> </nowiki>8.7
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| − | <nowiki> </nowiki>4.4
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| − | <nowiki> </nowiki>2.18
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| − | <nowiki> </nowiki>'''5'''
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| − | <nowiki> </nowiki>NaNO<sub>3</sub>
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| − | <nowiki> </nowiki>85.01
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| − | <nowiki> </nowiki>85.0
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| − | <nowiki> </nowiki>42.5
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| − | <nowiki> </nowiki>21.25
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| − | |-
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| − | <nowiki> </nowiki>'''6'''
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| − |
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| − | |
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| − | <nowiki> </nowiki>NaSiO<sub>3</sub> · 5 H<sub>2</sub>O
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| − |
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| − | |
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| − | <nowiki> </nowiki>212.16
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| − | <nowiki> </nowiki>21.2
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| − | <nowiki> </nowiki>10.6
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| − | <nowiki> </nowiki>5.30
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| − | <nowiki> </nowiki>''' '''
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| − | <nowiki> </nowiki>'''Trace metals'''
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| − | <nowiki> </nowiki>[M] g/mol
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| − | <nowiki> </nowiki>mg/L
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| − | <nowiki> </nowiki>mg/500 mL
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| − | <nowiki> </nowiki>mg/250 mL
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| − |
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| − | |-
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| − | <nowiki> </nowiki>Na<sub>2</sub> · EDTA
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>372.24
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| − |
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| − | |
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| − | <nowiki> </nowiki>4360
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| − | <nowiki> </nowiki>2 180
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| − | <nowiki> </nowiki>1 090.0
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| − |
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| − | |-
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| − | |
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| − |
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| − | |
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| − | <nowiki> </nowiki>FeCl<sub>3</sub> · 6 H<sub>2</sub>O
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| − |
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| − | |
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| − | <nowiki> </nowiki>270.32
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| − |
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| − | |
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| − | <nowiki> </nowiki>315
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| − |
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| − | |
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| − | <nowiki> </nowiki>1 575
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| − | <nowiki> </nowiki>787.5
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| − |
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| − | |-
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| − | |
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| − |
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| − | |
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| − | <nowiki> </nowiki>CuSO<sub>4</sub> · 5 H<sub>2</sub>O
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>249.69
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| − |
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| − | |
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| − | <nowiki> </nowiki>10
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| − |
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| − | |
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| − | <nowiki> </nowiki>5
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| − |
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| − | <nowiki> </nowiki>2.5
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| − |
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| − | |-
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| − | |
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| − |
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| − | |
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| − | <nowiki> </nowiki>ZnSO<sub>4</sub> · 7 H<sub>2</sub>O
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>287.54
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>22
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>11
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| − |
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| − | |
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| − | <nowiki> </nowiki>5.5
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| − |
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| − | |-
| |
| − | |
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>CoCl<sub>2</sub> · 6 H<sub>2</sub>O
| |
| − |
| |
| − | |
| |
| − | <nowiki> </nowiki>237.93
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>10
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>5
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>2.5
| |
| − |
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| − | |-
| |
| − | |
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>MnCl<sub>2</sub> · 4 H2O
| |
| − |
| |
| − | |
| |
| − | <nowiki> </nowiki>197.90
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>180
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>90
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>45
| |
| − |
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| − | |-
| |
| − | |
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| − |
| |
| − | |
| |
| − | <nowiki> </nowiki>NaMoO<sub>4</sub> · 2 H<sub>2</sub>O
| |
| − |
| |
| − | |
| |
| − | <nowiki> </nowiki>241.95
| |
| − |
| |
| − | |
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| − | <nowiki> </nowiki>6
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>3
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>1.5
| |
| − |
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| − | |-
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| − | |
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| − |
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| − | |
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| − | <nowiki> </nowiki>H<sub>3</sub>BO<sub>3</sub>
| |
| − |
| |
| − | |
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| − | <nowiki> </nowiki>61.83
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| − |
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| − | |
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| − | <nowiki> </nowiki>1000
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>500
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| − |
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| − | |
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| − | <nowiki> </nowiki>250.0
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| − |
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| − | |-
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| − | |-
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| − | <nowiki> </nowiki>'''8'''
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| − |
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| − | |
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| − | <nowiki> </nowiki>'''Vitamins''' | |
| − |
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| − | |
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| − | <nowiki> </nowiki>[M] g/mol
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>mg/L
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>mg/500 mL
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| − |
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| − | |
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| − | <nowiki> </nowiki>mg/250 mL
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| − |
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| − | |-
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| − | |
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| − |
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| − | |
| |
| − | <nowiki> </nowiki>Thiamin · HCl
| |
| − |
| |
| − | |
| |
| − | <nowiki> </nowiki>337.30
| |
| − |
| |
| − | |
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| − | <nowiki> </nowiki>100.0
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>50.00
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>25.000
| |
| − |
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| − | |-
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| − | |
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| − |
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| − | |
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| − | <nowiki> </nowiki>Biotin
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>244.31
| |
| − |
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| − | |
| |
| − | <nowiki> </nowiki>0.5
| |
| − |
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| − | |
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| − | <nowiki> </nowiki>0.25
| |
| − |
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| − | |
| |
| − | <nowiki> </nowiki>0.125
| |
| − |
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| − | |-
| |
| − | |
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| − |
| |
| − | |
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| − | <nowiki> </nowiki>B<sub>12</sub>
| |
| − |
| |
| − | |
| |
| − | <nowiki> </nowiki>1355.38
| |
| − |
| |
| − | |
| |
| − | <nowiki> </nowiki>0.5
| |
| − |
| |
| − | |
| |
| − | <nowiki> </nowiki>0.25
| |
| − |
| |
| − | |
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| − | <nowiki> </nowiki>0.125
| |
| − |
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| − | |}
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| − | | |
| − | | |
| − | | |
| | == References == | | == References == |
Dissolve TES buffer in Type I purified water (e.g., Milli-Q) using a magnetic stirrer and add 1 mL/L of stock solutions and trace metals in numerical order (1–7). Autoclave appropriately and add 1 mL/L vitamins (8) through a sterile 0.22 μm polystyrene (PES) filter once cooled down to room temperature.
