WC

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Description

The WC (Wright Chu) medium is a commonly used fully-defined medium for culturing of limnic algae. It was first described by Guillard & Lorenzen (1972), and the most-commonly used recipe includes modifications as reported by Makulla (2000).[1][2]

Recipe

Dissolve TES buffer in Type I purified water (e.g., Milli-Q) using a magnetic stirrer and add 1 mL/L of stock solutions and trace metals in numerical order (1–7). Autoclave appropriately and add 1 mL/L vitamins (8) through a sterile 0.22 μm polystyrene (PES) filter once cooled down to room temperature.

Buffer
Compound TES
[M] g/mol 229.25
mg/5 L 575
mg/L 115
Stock solution 1 2 3 4 5 6
Compound CaCl2 · 2 H2O MgSO4 · 7 H2O NaHCO3 K2HPO4 NaNO3 NaSiO3 · 5 H2O
[M] g/mol
g/L
g/500 mL
g/250 mL
Stock solution 7: Trace metals
Compound Na2 · EDTA FeCl3 · 6 H2O CuSO4 · 5 H2O ZnSO4 · 7 H2O CoCl2 · 6 H2O MnCl2 · 4 H2O NaMoO4 · 2 H2O H3BO3
[M] g/mol
g/L
g/500 mL
g/250 mL
Stock solution 8: Vitamins
Compound Thiamin · HCl Biotin B12
[M] g/mol
g/L
g/500 mL
g/250 mL

Prepare stock solutions, trace metals, and vitamins in Type I purified water. Stock solution 4 (K2HPO4) should not be kept in in a polyethylene (PE) bottle and stock solution 6 (NaSiO3 · 5 H2O) should be kept in a polystyrene (PS) bottle instead of a quartz glass bottle.[3][4] For vitamins, prepare 1 mL aliquots and keep at -20 °C; thaw to use.

References

  1. Guillard, R.R.L. and Lorenzen, C.J. (1972) Yellow-green algae with chlorophyllide c. Journal of Phycology, 8:1, 10–18. DOI: 10.1111/j.1529-8817.1972.tb03995.x
  2. Makulla, A. (2000) Fatty acid composition of Scenedesmus obliquus: Correlation to dilution rates. Limnologica, 30:2, 162–168. DOI: 10.1016/S0075-9511(00)80011-0
  3. Hassenteufel, W., Jagitsch, R. & Koczy, F.F. (1963) Impregnation of glass surface against sorption of phosphate traces. Limnology and Oceanography, 8:2, 152–156. DOI: 10.4319/lo.1963.8.2.0152
  4. Harrison, P.J. & Berges, J.A. (2005) Marine culture media. In: Algal culturing techniques (ed. R.A. Andersen), 1st edition, 596 p. Elsevier Academic Press; Burlington, MA, USA.