Difference between revisions of "Main Page"

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= BLAST =
+
= BLAST for dummies =
  
test
+
== Sequence similarity searches: queries and hits ==
  
== BLAST for dummies ==
+
== The BLAST database ==
  
test
+
== Aligning query and hit sequences ==
  
== BLAST on the UoO Abel HPC ==
+
== Understanding the BLAST output ==
  
test
+
= Measuring BLAST hits =
  
= PacBio bioinformatics =
+
== Similarity: bitscores ==
  
[[BLAST for dummies|BLAST for dummies]]
+
== Significance: E-values ==
  
 +
== Similarity vs. significance ==
  
 +
= blastn vs. blastp =
  
-[[Primer]]
+
= Similarity, homology and orthology =
  
<br/>-[[Sequencing]]
+
= Improving BLAST hits =
  
--[[Assembly]]
+
== Adjusting BLAST search parameters ==
  
--[[Read mapping]]
+
== Reducing the database size ==
  
--[[Amplicon sequencing]]
+
== Increasing the quality of the alignment ==
  
<br/>-[[Phylogenetics]]
+
== Using addition positional information ==
  
--[[Alignments]]
+
== Adding query sequences ==
  
--[[Phylogenetic trees]]
+
= How to run BLAST =
  
-[[Diversity Analysis]]
+
== Running BLAST on the NCBI website ==
  
<br/>-[[Sequence similarity searches]]
+
== Running BLAST on the UoO Lifeportal ==
  
--[[BLAST]]
+
== Running BLAST manually from the command line ==
  
--[[HMMER]]
+
= Computer directories and the PATH =
  
--[[INFERNAL]]
+
== The current directory ==
 +
 
 +
== The PATH ==
 +
 
 +
= Running BLAST from the command-line =
 +
 
 +
== A simple BLAST search ==
 +
 
 +
== Creating a custom BLAST database ==
 +
 
 +
= General tips for using BLAST on Abel =
 +
 
 +
== Submit a BLAST search job script ==
 +
 
 +
== Use “qlogin” to directly interact with Abel during job execution ==
 +
 
 +
== Use the “freebee” system ==

Revision as of 12:13, 23 February 2015

BLAST for dummies

Sequence similarity searches: queries and hits

The BLAST database

Aligning query and hit sequences

Understanding the BLAST output

Measuring BLAST hits

Similarity: bitscores

Significance: E-values

Similarity vs. significance

blastn vs. blastp

Similarity, homology and orthology

Improving BLAST hits

Adjusting BLAST search parameters

Reducing the database size

Increasing the quality of the alignment

Using addition positional information

Adding query sequences

How to run BLAST

Running BLAST on the NCBI website

Running BLAST on the UoO Lifeportal

Running BLAST manually from the command line

Computer directories and the PATH

The current directory

The PATH

Running BLAST from the command-line

A simple BLAST search

Creating a custom BLAST database

General tips for using BLAST on Abel

Submit a BLAST search job script

Use “qlogin” to directly interact with Abel during job execution

Use the “freebee” system