Difference between revisions of "Main Page"

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= BLAST for dummies =
+
= BLAST: the Basic Local Alignment Search Tool =
  
== Sequence similarity searches: queries and hits ==
+
== BLAST for dummies ==
  
== The BLAST database ==
+
=== Sequence similarity searches: queries and hits ===
  
== Aligning query and hit sequences ==
+
=== The BLAST database ===
  
== Understanding the BLAST output ==
+
=== Aligning query and hit sequences ===
  
= Measuring BLAST hits =
+
=== Understanding the BLAST output ===
  
== Similarity: bitscores ==
+
== Measuring BLAST hits ==
  
== Significance: E-values ==
+
=== Similarity: bitscores ===
  
== Similarity vs. significance ==
+
=== Significance: E-values ===
  
= blastn vs. blastp =
+
=== Similarity vs. significance ===
  
= Similarity, homology and orthology =
+
== blastn vs. blastp ==
  
= Improving BLAST hits =
+
== Similarity, homology and orthology ==
  
== Adjusting BLAST search parameters ==
+
== Improving BLAST hits ==
  
== Reducing the database size ==
+
=== Adjusting BLAST search parameters ===
  
== Increasing the quality of the alignment ==
+
=== Reducing the database size ===
  
== Using addition positional information ==
+
=== Increasing the quality of the alignment ===
  
== Adding query sequences ==
+
=== Using addition positional information ===
  
= How to run BLAST =
+
=== Adding query sequences ===
  
== Running BLAST on the NCBI website ==
+
== How to run BLAST ==
  
== Running BLAST on the UoO Lifeportal ==
+
=== Running BLAST on the NCBI website ===
  
== Running BLAST manually from the command line ==
+
=== Running BLAST on the UoO Lifeportal ===
  
= Computer directories and the PATH =
+
=== Running BLAST manually from the command line ===
  
== The current directory ==
+
== Computer directories and the PATH ==
  
== The PATH ==
+
=== The current directory ===
  
= Running BLAST from the command-line =
+
=== The PATH ===
  
== A simple BLAST search ==
+
== Running BLAST from the command-line ==
  
== Creating a custom BLAST database ==
+
=== A simple BLAST search ===
  
= General tips for using BLAST on Abel =
+
=== Creating a custom BLAST database ===
  
== Submit a BLAST search job script ==
+
== General tips for using BLAST on Abel ==
  
== Use “qlogin” to directly interact with Abel during job execution ==
+
=== Submit a BLAST search job script ===
  
== Use the “freebee” system ==
+
=== Use “qlogin” to directly interact with Abel during job execution ===
 +
 
 +
=== Use the “freebee” system ===

Revision as of 12:16, 23 February 2015

BLAST: the Basic Local Alignment Search Tool

BLAST for dummies

Sequence similarity searches: queries and hits

The BLAST database

Aligning query and hit sequences

Understanding the BLAST output

Measuring BLAST hits

Similarity: bitscores

Significance: E-values

Similarity vs. significance

blastn vs. blastp

Similarity, homology and orthology

Improving BLAST hits

Adjusting BLAST search parameters

Reducing the database size

Increasing the quality of the alignment

Using addition positional information

Adding query sequences

How to run BLAST

Running BLAST on the NCBI website

Running BLAST on the UoO Lifeportal

Running BLAST manually from the command line

Computer directories and the PATH

The current directory

The PATH

Running BLAST from the command-line

A simple BLAST search

Creating a custom BLAST database

General tips for using BLAST on Abel

Submit a BLAST search job script

Use “qlogin” to directly interact with Abel during job execution

Use the “freebee” system