Difference between revisions of "Quality control of fastq read files"

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(Created page with "The first step of any analysis involving high-throughput sequencing data should consist of assessing the quality of the read data, possibly followed by the removal of low-qua...")
 
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It is possible to filter sequencing reads using Qiime, but a comprehensive report is not produced in the process. The FastQC program may be used to produce such a report, optionally followed by read filtering using the Trimmomatic program.
 
It is possible to filter sequencing reads using Qiime, but a comprehensive report is not produced in the process. The FastQC program may be used to produce such a report, optionally followed by read filtering using the Trimmomatic program.
  
See here for an overview.
+
See [https://wiki.uio.no/mn/ibv/bioinfwiki/index.php/RNASeq:_Quality_control here] for an overview.
  
  
 
https://wiki.uio.no/mn/ibv/bioinfwiki/index.php/RNASeq:_Quality_control
 
https://wiki.uio.no/mn/ibv/bioinfwiki/index.php/RNASeq:_Quality_control

Revision as of 12:57, 24 August 2015

The first step of any analysis involving high-throughput sequencing data should consist of assessing the quality of the read data, possibly followed by the removal of low-quality reads.


It is possible to filter sequencing reads using Qiime, but a comprehensive report is not produced in the process. The FastQC program may be used to produce such a report, optionally followed by read filtering using the Trimmomatic program.

See here for an overview.


https://wiki.uio.no/mn/ibv/bioinfwiki/index.php/RNASeq:_Quality_control