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== CEES metagenomics wiki ==
 
== CEES metagenomics wiki ==
  
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== Protocols ==
 
== Protocols ==
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DNA extraction and 16S rDNA amplification are performed according to the protocols of the [http://www.earthmicrobiome.org/ Earth MIcrobiome Project].
  
 
=== DNA extraction ===
 
=== DNA extraction ===

Revision as of 10:28, 5 March 2015

CEES metagenomics wiki

This wiki is meant as a resource for people performing metagenomics work in CEES. The website contains protocols for DNA extraction, PCR, library preps, sequencing etc., in addition to relevant literature.

Tips for adding a page (from the bioinf-wiki of Lex).

Material

The starting material currently includes cod gut samples, human stool samples and (...?).

Protocols

DNA extraction and 16S rDNA amplification are performed according to the protocols of the Earth MIcrobiome Project.

DNA extraction

For the above mentioned materials, the main procedure for DNA extraction is a modified version of the DNeasy Blood & Tissue Kit, including an initial bead beating step.

DNeasy 96 Blood & Tissue Kit handbook

Paper describing the modified protocol: "High-throughput extraction of arthropod vector and pathogen DNA using bead milling" (Allender et al. 2004)