Difference between revisions of "Yield of Tc in Liquid-Liquid Extraction"
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| + | <br> In this exercise you will learn how to use a radionuclide to measure a feature of an element, in addition to how radionuclides can be used as a tracer to study a chemical reaction. | ||
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| + | The exercise will show how Tc can be extracted from an aquatic phase by using liquid-liquid extraction. It will illustrate how easy it is to follow a radioactive element during a reaction. Therefore tracers are used in a lot of different tests in several branches of industry and academics. | ||
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| + | To a 15 mL test tube add 2.5 mL 0.1 M HNO<sub>3</sub> / 0.05 M KBrO<sub>3</sub> solution. Then add 0.5 mL of <sup>99m</sup>Tc solution. Then add 3.0 mL 0.05 M trioctylamin dissolved in toluene. Shake the solution vigorously for one minute. Transfer 2 mL of each phase to a glass vial. ''Be precise so that you don't mix the phases in the vial''. It is often better to transfer 2 mL of the upper phase, then remove the rest of it. Then transfer 2 mL of the bottom phase. | ||
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| + | The amount <sup>99m</sup>Tc in the two phases is decided by quantitative analysis of the <span class="texhtml">γ</span> spectrum. Measure the spectrums and write down the following: <br> Counts in organic phase, N<sub>org</sub> (“Net Area”): …..........................<br> Counting time for organic phase, t<sub>org</sub>(“Live Time”):....................sec<br> Counting speed for organic phase R<sub>org</sub>(N<sub>org</sub>/t<sub>org</sub>):....................cps<br> <br><br> Counts in water phase, N<sub>aq</sub> (“Net Area”):.........................<br> Counting time for water phase, t<sub>aq</sub> (“Live time”):..................sec<br> Counting speed for water phase R<sub>aq</sub>(N<sub>aq</sub>/t<sub>aq</sub>):....................cps<br> Distribution, D=R<sub>org</sub>/R<sub>aq</sub>:...................<br> Extraction yield, E=R<sub>org</sub>/(R<sub>org</sub>+R<sub>aq</sub>)<math>\cdot</math>100............... % | ||
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| + | [[Category:Nuclear_Properties]] [[Category:Laboratory_exercise]] [[Category:Radio_chemistry]] [[Category:Radionuclide_generator]] [[Category:Liquid-Liquid_Extraction]] | ||
Latest revision as of 16:25, 28 June 2012
Return to Main
In this exercise you will learn how to use a radionuclide to measure a feature of an element, in addition to how radionuclides can be used as a tracer to study a chemical reaction.
The exercise will show how Tc can be extracted from an aquatic phase by using liquid-liquid extraction. It will illustrate how easy it is to follow a radioactive element during a reaction. Therefore tracers are used in a lot of different tests in several branches of industry and academics.
To a 15 mL test tube add 2.5 mL 0.1 M HNO3 / 0.05 M KBrO3 solution. Then add 0.5 mL of 99mTc solution. Then add 3.0 mL 0.05 M trioctylamin dissolved in toluene. Shake the solution vigorously for one minute. Transfer 2 mL of each phase to a glass vial. Be precise so that you don't mix the phases in the vial. It is often better to transfer 2 mL of the upper phase, then remove the rest of it. Then transfer 2 mL of the bottom phase.
The amount 99mTc in the two phases is decided by quantitative analysis of the γ spectrum. Measure the spectrums and write down the following:
Counts in organic phase, Norg (“Net Area”): …..........................
Counting time for organic phase, torg(“Live Time”):....................sec
Counting speed for organic phase Rorg(Norg/torg):....................cps
Counts in water phase, Naq (“Net Area”):.........................
Counting time for water phase, taq (“Live time”):..................sec
Counting speed for water phase Raq(Naq/taq):....................cps
Distribution, D=Rorg/Raq:...................
Extraction yield, E=Rorg/(Rorg+Raq)100............... %
