Difference between revisions of "Practical Exercise For Liquid Scintillation"

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#Determination of the counting efficiency of 3H and 14C<br> * Prepare one standard sample (unquenched) for each of the two radionuclides 3H and 14C in two separate liquid scintillation vials. This is done by extracting an aliquote of 1.00 mL from the respective mother solutions organized by the laboratory assistant into the two vials. Add 10 ml scintillation cocktail to each vial and shake to a homogeneous solution. b. Count the standard samples on the Beckman LS counter in the MCA mode. Define channel 1 as the counting window covering the 3H spectrum, and channel 2 as that part of the 14C spectrum which does not overlap with the 3H spectrum. For 14C record the counting rate in both channels. c. Determine the counting efficiency CH1(3H), CH1(14C), CH2(14C) and CH1+CH2(14C) from Eqn.2. d. Record (plot) the scintillation spectra for the two radionuclides.
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#Determination of the counting efficiency of <sup>3</sup>H and <sup>14</sup>C<br>a. Prepare one standard sample (unquenched) for each of the two radionuclides <sup>3</sup>H and <sup>14</sup>C in two separate liquid scintillation vials. This is done by extracting an aliquote of 1.00 mL from the respective mother solutions organized by the laboratory assistant into the two vials. Add 10 ml scintillation cocktail to each vial and shake to a homogeneous solution. <br>b. Count the standard samples on the Beckman LS counter in the MCA mode. Define channel 1 as the counting window covering the <sup>3</sup>H spectrum, and channel 2 as that part of the <sup>14</sup>C spectrum which does not overlap with the <sup>3</sup>H spectrum. For <sup>14</sup>C record the counting rate in both channels.<br>c. Determine the counting efficiency CH1(<sup>3</sup>H), CH1(<sup>14</sup>C), CH2(<sup>14</sup>C) and CH1+CH2(<sup>14</sup>C) from Eqn.2. <br>d. Record (plot) the scintillation spectra for the two radionuclides.

Revision as of 09:40, 20 June 2012

The students are divided first into two groups. Each group follows the procedure below.

Laboratory Procedure
  1. Determination of the counting efficiency of 3H and 14C
    a. Prepare one standard sample (unquenched) for each of the two radionuclides 3H and 14C in two separate liquid scintillation vials. This is done by extracting an aliquote of 1.00 mL from the respective mother solutions organized by the laboratory assistant into the two vials. Add 10 ml scintillation cocktail to each vial and shake to a homogeneous solution.
    b. Count the standard samples on the Beckman LS counter in the MCA mode. Define channel 1 as the counting window covering the 3H spectrum, and channel 2 as that part of the 14C spectrum which does not overlap with the 3H spectrum. For 14C record the counting rate in both channels.
    c. Determine the counting efficiency CH1(3H), CH1(14C), CH2(14C) and CH1+CH2(14C) from Eqn.2.
    d. Record (plot) the scintillation spectra for the two radionuclides.
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