KJM5911 Lab Exercise 5 - Liquid Scintillation Counting
- Understand the theory behind Liquid Scintillation detection.
- Understand how the detection equipment in Liquid Scintillation works.
We are going to use a modern liquid-scintillation counter (LSC) with three PM-tubes. This enables it to use the Triple-to-Doubble-Coincidence-Ratio (TDCR) parameter for quench correction. We will both use the traditional channel-ratio method and the TDCR method to investigate quenching in LSC samples. For this we will use 14C samples quenched with CCl.
Part 1: Initial tests
- Measure 3T and 14C standards to get familiar with the machine (HIDEX 300) (2 min each).
- Measure an empty vial with 10 mL scintillator (and nothing else) inside (10 min or more).
- Measure an empty vial without scintillator solution (10 min or more).
- HIDEX 300 Automatic TDCR Liquid Scintillation Counter