KJM5911 Lab Exercise 5 - Liquid Scintillation Counting

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Learning Goals

  • Understand the theory behind Liquid Scintillation detection.
  • Understand how the detection equipment in Liquid Scintillation works.

Theory

Experimental procedure

We are going to use a modern liquid-scintillation counter (LSC) with three PM-tubes. This enables it to use the Triple-to-Doubble-Coincidence-Ratio (TDCR) parameter for quench correction. We will both use the traditional channel-ratio method and the TDCR method to investigate quenching in LSC samples. For this we will use 14C samples quenched with CCl.

Part 1: Initial tests

  • Measure 3T and 14C standards to get familiar with the machine (HIDEX 300) (2 min each).
  • Measure an empty vial with 10 mL scintillator (and nothing else) inside (10 min or more).
  • Measure an empty vial without scintillator solution (10 min or more).

Equipment

  • HIDEX 300 Automatic TDCR Liquid Scintillation Counter

Safety Aspects