Difference between revisions of "Laboratory Exercise - Safe Working Practise in the Radionuclide Laboratory and Preparation of Counting Samples"

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(Experimental procedure)
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=== Equipment  ===
=== Equipment  ===
*HIDEX 300 Automatic TDCR Liquid Scintillation Counter
=== Safety Aspects  ===
=== Safety Aspects  ===

Latest revision as of 19:51, 21 November 2012

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Learning Goals

After having performed this exercise you should:

  • Understand the basics of practical radiation protection.
  • Know how to prepare typical counting samples for radiation measurements.


Experimental procedure

The objective of the exercise is to learn safe handling of radionuclide-containing solutions and to prepare counting samples for measurement in the following radiochemistry laboratory course. Perform the exercise on a tray, and always keep absorbent cellulose available. Wear gloves when pipetting. Use an adjustable automatic pipette (Finnpipette, Biohit) and change tips between different solutions. Under no circumstances contaminate the pipette by turning it so that the tip is uppermost.

Preparation of 32P samples

  1. The activity concentration of the 32P stock solution used in this exercise is XXX kBq/mL. It also contains carrier phosphate of concentration ~10-4 M.
  2. Take a tray for your group and cut some protective paper (shiny side up) to cover its upper, inner surface. Draw a grid on the paper, and mark the columns A–D and the rows 1–5.
  3. Take five 20 mL plastic vials and mark them 1, 2,.., 5. To each vial, pipette 4 mL gelatin solution (0.2% gelatine, 10-4 M phosphate, 0.03% sodium dodecyl sulphate and 2∙10-2 M sodium hydroxide). Pipette 1, 2,.., 5 mL 32P stock solution into vials 1, 2,.., 5, respectively. Fill each vial with water so that their volumes are all 10 mL, close their caps and shake them gently ten times (vigorous shaking will cause undesirable foaming).
  4. Make four parallel counting samples for each 32P dilution: Take twenty aluminium discs and mark their reverse sides 1A, 1B,.., 5D. Place the discs in their designated positions on the tray. Pipette 0.2 mL solution from vial 1 to each disc in row 1, and proceed similarly for rows 2–5.
  5. Use a surface contamination meter to make a few control measurements. The instructor will direct you in correct procedure.
  6. Leave the samples in a fume cupboard to dry overnight. The samples will be used in Basic radiochemistry laboratory exercise course.
  7. Dispose of your 32P dilutions accordingly. The dilutions and first washings of the vials go into the waste container. Rinse the vials many times with tap water and then dispose of them in the normal waste.

Detection of surface contamination

The teacher has evaporated one or several drops of 134Cs, emitting both  and  radiation, solution on a table having 25 blocks. The has coordinates 1-5 on the other edge and A-E on the other. The blocks containing the "contamination" should be detected with a PCM 5/1 detector. Turn on the detector, and switch Electra to "" mode and write down the high voltage. Test the detector with a calibration source given by the teacher.

Filling in the exercise form

Fill in the exercise report form provided, and submit it within two weeks after completion of the exercise.


Safety Aspects