Difference between revisions of "Yield of Tc in Liquid-Liquid Extraction"

From mn/safe/nukwik
Jump to: navigation, search
 
Line 1: Line 1:
 
Return to [[Basic Laboratory Procedures for Radio-Chemistry|Main]]  
 
Return to [[Basic Laboratory Procedures for Radio-Chemistry|Main]]  
  
<br> In this exercise you will learn how you can use a radionuclide to measure a feature of a element. In addition how radionuclides can be used as a tracer to study a chemical reaction.  
+
<br> In this exercise you will learn how to use a radionuclide to measure a feature of an element, in addition to how radionuclides can be used as a tracer to study a chemical reaction.  
  
The exercise will show how Tc can be extracted from a aquatic phase by using liquid-liquid extraction. It will illustrate how easy it is to follow a radioactive element during a reaction. Therefore tracers are used in a lot of different tests in several branches of industry and academics.  
+
The exercise will show how Tc can be extracted from an aquatic phase by using liquid-liquid extraction. It will illustrate how easy it is to follow a radioactive element during a reaction. Therefore tracers are used in a lot of different tests in several branches of industry and academics.  
  
To a 15mL test tube add 2.5 mL 0.1 M HNO<sub>3</sub> / 0.05 M KbrO<sub>3</sub> solution. Then add 0.5 mL of <sup>99m</sup>Tc solution. Then add 3.0 mL 0.05 M trioctylamin dissolved in toluene. Shake the solution vigorously fro one minute. Transfer 2 mL of each phase to a glass vial. ''Be precise so that you don't mix the phases in the vial''. It is often better to transfer 2mL of the upper phase, then remove the rest of it. Then transfer 2 mL of the bottom phase.  
+
To a 15 mL test tube add 2.5 mL 0.1 M HNO<sub>3</sub> / 0.05 M KBrO<sub>3</sub> solution. Then add 0.5 mL of <sup>99m</sup>Tc solution. Then add 3.0 mL 0.05 M trioctylamin dissolved in toluene. Shake the solution vigorously for one minute. Transfer 2 mL of each phase to a glass vial. ''Be precise so that you don't mix the phases in the vial''. It is often better to transfer 2 mL of the upper phase, then remove the rest of it. Then transfer 2 mL of the bottom phase.  
  
 
The amount <sup>99m</sup>Tc in the two phases is decided by quantitative analysis of the <span class="texhtml">γ</span> spectrum. Measure the spectrums and write down the following: <br> Counts in organic phase, N<sub>org</sub> (“Net Area”): …..........................<br> Counting time for organic phase, t<sub>org</sub>(“Live Time”):....................sec<br> Counting speed for organic phase R<sub>org</sub>(N<sub>org</sub>/t<sub>org</sub>):....................cps<br> <br><br> Counts in water phase, N<sub>aq</sub> (“Net Area”):.........................<br> Counting time for water phase, t<sub>aq</sub> (“Live time”):..................sec<br> Counting speed for water phase R<sub>aq</sub>(N<sub>aq</sub>/t<sub>aq</sub>):....................cps<br> Distribution, D=R<sub>org</sub>/R<sub>aq</sub>:...................<br> Extraction yield, E=R<sub>org</sub>/(R<sub>org</sub>+R<sub>aq</sub>)<math>\cdot</math>100...............&nbsp;%  
 
The amount <sup>99m</sup>Tc in the two phases is decided by quantitative analysis of the <span class="texhtml">γ</span> spectrum. Measure the spectrums and write down the following: <br> Counts in organic phase, N<sub>org</sub> (“Net Area”): …..........................<br> Counting time for organic phase, t<sub>org</sub>(“Live Time”):....................sec<br> Counting speed for organic phase R<sub>org</sub>(N<sub>org</sub>/t<sub>org</sub>):....................cps<br> <br><br> Counts in water phase, N<sub>aq</sub> (“Net Area”):.........................<br> Counting time for water phase, t<sub>aq</sub> (“Live time”):..................sec<br> Counting speed for water phase R<sub>aq</sub>(N<sub>aq</sub>/t<sub>aq</sub>):....................cps<br> Distribution, D=R<sub>org</sub>/R<sub>aq</sub>:...................<br> Extraction yield, E=R<sub>org</sub>/(R<sub>org</sub>+R<sub>aq</sub>)<math>\cdot</math>100...............&nbsp;%  
  
[[Category:Nuclear_Properties]][[Category:Laboratory_exercise]][[Category:Radio_chemistry]][[Category:Radionuclide_generator]][[Category:Liquid-Liquid Extraction]]
+
[[Category:Nuclear_Properties]] [[Category:Laboratory_exercise]] [[Category:Radio_chemistry]] [[Category:Radionuclide_generator]] [[Category:Liquid-Liquid_Extraction]]

Latest revision as of 15:25, 28 June 2012

Return to Main


In this exercise you will learn how to use a radionuclide to measure a feature of an element, in addition to how radionuclides can be used as a tracer to study a chemical reaction.

The exercise will show how Tc can be extracted from an aquatic phase by using liquid-liquid extraction. It will illustrate how easy it is to follow a radioactive element during a reaction. Therefore tracers are used in a lot of different tests in several branches of industry and academics.

To a 15 mL test tube add 2.5 mL 0.1 M HNO3 / 0.05 M KBrO3 solution. Then add 0.5 mL of 99mTc solution. Then add 3.0 mL 0.05 M trioctylamin dissolved in toluene. Shake the solution vigorously for one minute. Transfer 2 mL of each phase to a glass vial. Be precise so that you don't mix the phases in the vial. It is often better to transfer 2 mL of the upper phase, then remove the rest of it. Then transfer 2 mL of the bottom phase.

The amount 99mTc in the two phases is decided by quantitative analysis of the γ spectrum. Measure the spectrums and write down the following:
Counts in organic phase, Norg (“Net Area”): …..........................
Counting time for organic phase, torg(“Live Time”):....................sec
Counting speed for organic phase Rorg(Norg/torg):....................cps


Counts in water phase, Naq (“Net Area”):.........................
Counting time for water phase, taq (“Live time”):..................sec
Counting speed for water phase Raq(Naq/taq):....................cps
Distribution, D=Rorg/Raq:...................
Extraction yield, E=Rorg/(Rorg+Raq)[math]\cdot[/math]100............... %