Difference between revisions of "CrossSeeds"

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== About ==
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=== In the CrossSeeds project, our working hypothesis that AD, PD and HD share common pathways of protein aggregation and that cross-seeding of pathogenic pGlu-modified proteins are leading to neurodegeneration. On this page we show examples of pGlu-protein expression sites in rat and mouse models allowing visualization in 3D reference atlas space (Waxholm rat brain atlas and Allen brain mouse atlas). A database in preparation will allow storage of all raw data, retrieval and dissemination of findings to the scientific community. ===
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== Access image repository ==
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=== The virtual microscopy filmstrip viewer allows interactive zooming and panning of images. The Localizoom link allows to visualize the sections together with customized reference atlas overlays. ===
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== AD mouse tg2576 ==
 
== AD mouse tg2576 ==
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This 18 month old Alzheimer’s mouse was labelled with 4 different antibodies against human APP (1D1), against APP and Abeta (4G8), against Abeta but not APP (MOAB-2) and against pGlu-Abeta.
  
 
=== 1D1 ===
 
=== 1D1 ===
 
[http://cmbn-navigator.uio.no/navigator/filmstrip_viewer_custom.html?representationList=47927,47943,47965 filmstrip]
 
[http://cmbn-navigator.uio.no/navigator/filmstrip_viewer_custom.html?representationList=47927,47943,47965 filmstrip]
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* 761_2222_2904_Mtg01_bl1_1D1_s014 [http://cmbn-navigator.uio.no/navigator/alignii_tool.html?sectionId=47927&atlasDescriptorId=100000&ox=418.8840842422267&oy=293.4996854837767&oz=298.5794086268716&ux=-385.98392179034664&uy=20.413938415529454&uz=-1.6007071470477856&vx=2.9379052380620583&vy=31.765660366701244&vz=-303.31699767461936 alignii]
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* 761_2222_2904_Mtg01_bl1_1D1_s014 [http://folk.uio.no/gergelyc/ABAZoomer/zoomer.html?id=47927&ox=418.8840842422267&oy=293.4996854837767&oz=298.5794086268716&ux=-385.98392179034664&uy=20.413938415529454&uz=-1.6007071470477856&vx=2.9379052380620583&vy=31.765660366701244&vz=-303.31699767461936 Localizoom]
 
* 761_2222_2904_Mtg01_bl1_1D1_s028 [http://cmbn-navigator.uio.no/navigator/alignii_tool.html?sectionId=47943&atlasDescriptorId=100000&ox=428.5954687616411&oy=232.21300244225012&oz=323.0965220377168&ux=-405.3710573322881&uy=20.577177539897775&uz=6.341337728923577&vx=-3.462245875611303&vy=35.651269930982295&vz=-337.0103101567657 alignii]
 
* 761_2222_2904_Mtg01_bl1_1D1_s028 [http://cmbn-navigator.uio.no/navigator/alignii_tool.html?sectionId=47943&atlasDescriptorId=100000&ox=428.5954687616411&oy=232.21300244225012&oz=323.0965220377168&ux=-405.3710573322881&uy=20.577177539897775&uz=6.341337728923577&vx=-3.462245875611303&vy=35.651269930982295&vz=-337.0103101567657 alignii]
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* 761_2222_2904_Mtg01_bl1_1D1_s041 [http://cmbn-navigator.uio.no/navigator/alignii_tool.html?sectionId=47965&atlasDescriptorId=100000&ox=452.3239271912439&oy=159.70161887040172&oz=322.5266382784972&ux=-446.8082731539038&uy=22.214584769293083&uz=11.417213426918863&vx=-6.737555810166668&vy=35.34148447581845&vz=-332.4360090529058 alignii]
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* 761_2222_2904_Mtg01_bl1_1D1_s041 [http://folk.uio.no/gergelyc/ABAZoomer/zoomer.html?id=47927&ox=418.8840842422267&oy=293.4996854837767&oz=298.5794086268716&ux=-385.98392179034664&uy=20.413938415529454&uz=-1.6007071470477856&vx=2.9379052380620583&vy=31.765660366701244&vz=-303.31699767461936 alignii]
  
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=== MOAB ===
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=== MOAB2 ===
 
[http://cmbn-navigator.uio.no/navigator/filmstrip_viewer_custom.html?representationList=49569,49550,49577 filmstrip]
 
[http://cmbn-navigator.uio.no/navigator/filmstrip_viewer_custom.html?representationList=49569,49550,49577 filmstrip]
 
* 761_2222_2942_Mtg01_bl1_MOAB2_s015 [http://cmbn-navigator.uio.no/navigator/alignii_tool.html?sectionId=49569&atlasDescriptorId=100000&ox=414.5986046826093&oy=311.38893437455613&oz=298.7002690820383&ux=-372.44864072963423&uy=19.31071668440001&uz=1.980916300209856&vx=0.0425135899158704&vy=32.44111202494844&vz=-308.254715954993 alignii]
 
* 761_2222_2942_Mtg01_bl1_MOAB2_s015 [http://cmbn-navigator.uio.no/navigator/alignii_tool.html?sectionId=49569&atlasDescriptorId=100000&ox=414.5986046826093&oy=311.38893437455613&oz=298.7002690820383&ux=-372.44864072963423&uy=19.31071668440001&uz=1.980916300209856&vx=0.0425135899158704&vy=32.44111202494844&vz=-308.254715954993 alignii]

Revision as of 15:24, 13 March 2017

About

In the CrossSeeds project, our working hypothesis that AD, PD and HD share common pathways of protein aggregation and that cross-seeding of pathogenic pGlu-modified proteins are leading to neurodegeneration. On this page we show examples of pGlu-protein expression sites in rat and mouse models allowing visualization in 3D reference atlas space (Waxholm rat brain atlas and Allen brain mouse atlas). A database in preparation will allow storage of all raw data, retrieval and dissemination of findings to the scientific community.

Access image repository 

The virtual microscopy filmstrip viewer allows interactive zooming and panning of images. The Localizoom link allows to visualize the sections together with customized reference atlas overlays.

AD mouse tg2576

This 18 month old Alzheimer’s mouse was labelled with 4 different antibodies against human APP (1D1), against APP and Abeta (4G8), against Abeta but not APP (MOAB-2) and against pGlu-Abeta.

1D1

filmstrip

  • 761_2222_2904_Mtg01_bl1_1D1_s014 Localizoom
  • 761_2222_2904_Mtg01_bl1_1D1_s028 alignii
  • 761_2222_2904_Mtg01_bl1_1D1_s041 alignii

MOAB2

filmstrip

  • 761_2222_2942_Mtg01_bl1_MOAB2_s015 alignii
  • 761_2222_2942_Mtg01_bl1_MOAB2_s091 alignii
  • 761_2222_2942_Mtg01_bl1_MOAB2_s143 alignii

pGlu

filmstrip

  • 761_2222_2943_Mtg01_bl1_pGlu_s032 alignii
  • 761_2222_2943_Mtg01_bl1_pGlu_s092 alignii
  • 761_2222_2943_Mtg01_bl1_pGlu_s144 alignii

4G8

filmstrip

  • 761_2222_2941_Mtg01_bl1_4G8_s026 alignii
  • 761_2222_2941_Mtg01_bl1_4G8_s090 alignii
  • 761_2222_2941_Mtg01_bl1_4G8_s154 alignii

TG Hd rat

filmstrip

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