Difference between revisions of "CrossSeeds"
m (Tag: Visual edit) |
m (Tag: Visual edit) |
||
| Line 10: | Line 10: | ||
== AD mouse tg2576 == | == AD mouse tg2576 == | ||
| − | Two 18 month old Alzheimer’s mice were labelled with 4 different antibodies: against human APP (1D1) (Höfling et al. 2016 Aging Cell), against Aβ peptide (aa 17-24) (BioLegend,Cat:4G8), against Aβ peptide (aa 1-4) but not APP (Cayman Chemicals, Cat:MOAB-2) and against Pyroglutamate-modified Aβ peptides (Aβ<sub>pE3</sub>) (pGlu)(Probiodrug). Images of sections labelled with each of the antibodies can be viewed with the filmstrip viewer link. The atlas overlayed sections are available using the LocaliZoom tool as outlined above. | + | Two 18 month old Alzheimer’s mice (Mtg01_bl1 and m-287) were labelled with 4 different antibodies: against human APP (1D1) (Höfling et al. 2016 Aging Cell), against Aβ peptide (aa 17-24) (BioLegend,Cat:4G8), against Aβ peptide (aa 1-4) but not APP (Cayman Chemicals, Cat:MOAB-2) and against Pyroglutamate-modified Aβ peptides (Aβ<sub>pE3</sub>) (pGlu)(Probiodrug). Images of sections labelled with each of the antibodies can be viewed with the filmstrip viewer link. The atlas overlayed sections are available using the LocaliZoom tool as outlined above. |
| − | One tg2576 female mouse (18 months) was labelled by triple immunofluorescence labelling with antibodies against Aβ<sub>pE3</sub> (J8) conj. with Cy2 (green); α-synuclein (C20R) conj. with Cy3 (orange); Aβ peptide (aa 17-24) (4G8) conjugated with Cy5 (red) and Hoest labelling of Nuclei (Dapi channel)(blue). The triple labelling shows localization of α-synuclein close to amyloid plaques, thereby comforting the CrossSeeding hypothesis. | + | One tg2576 female mouse (m-543;18 months) was labelled by triple immunofluorescence labelling with antibodies against Aβ<sub>pE3</sub> (J8) conj. with Cy2 (green); α-synuclein (C20R) conj. with Cy3 (orange); Aβ peptide (aa 17-24) (4G8) conjugated with Cy5 (red) and Hoest labelling of Nuclei (Dapi channel)(blue). The triple labelling shows localization of α-synuclein close to amyloid plaques, thereby comforting the CrossSeeding hypothesis. |
=== Human APP (1D1) === | === Human APP (1D1) === | ||
| Line 96: | Line 96: | ||
==HD F344tg rat== | ==HD F344tg rat== | ||
| − | + | Two transgenic female Huntington rats ( Tg3898 and Tg3876; 90 weeks) and two female Wild type Fisher rats (WT 3949 and WT3884; 90weeks old) were labelled with antibodies against Huntingtin: against 2B7 antibody (mouse monoclonal, recognizes the N-terminus of Htt. CHDI foundation); against 3B5H10 antibody against Human Huntingtin (N-terminal 1-171 amino acid fragment of human Huntingtin with a 68 glutamine stretch) (Absolute antibody, Cat: A00144-1.1); against S829 (recognize mutated Htt aggregates, exon 1 (53Q)hHtt; gift from Prof. G. Bates). | |
=== 3B5H10 === | === 3B5H10 === | ||
Revision as of 17:10, 3 January 2018
Contents
About
The CrossSeeds project hypothesis is that AD, PD and HD share common pathways of protein aggregation and that cross-seeding of pathogenic pGlu-modified proteins are leading to neurodegeneration. This site demonstrates ongoing efforts in mapping of the distribution of pGlu-protein expression sites in rat and mouse models. High resolution histological section images from whole brains are organized in a database and anchored to 3D reference atlas space (Waxholm rat brain atlas and Allen mouse brain mouse atlas). The data system in preparation allows storage of all raw data, data retrieval, and visualization and analysis in 3D space.
Image repository, viewing, and analysis
The virtual microscopy filmstrip viewer allows interactive zooming and panning of images.
The Localizoom viewer uses the atlas anchoring information and allows visualization of single section images with a transparent atlas-segmentation overlay, matching the location in the brain of the section image and cut at the custom angle of orientation of the histological section. Names of atlas structures are shown when pointing the mouse over the region of interest and 3D coordinates at the location of the cursor are shown real-time. An extended version of the LocaliZoom can harvest coordinates corresponding to selected features of interest in the images for further 3D analysis. Zooming in a specific area is possible using the mouse wheel. Transparency of the atlas is adjustable on the top left corner scale.
AD mouse tg2576
Two 18 month old Alzheimer’s mice (Mtg01_bl1 and m-287) were labelled with 4 different antibodies: against human APP (1D1) (Höfling et al. 2016 Aging Cell), against Aβ peptide (aa 17-24) (BioLegend,Cat:4G8), against Aβ peptide (aa 1-4) but not APP (Cayman Chemicals, Cat:MOAB-2) and against Pyroglutamate-modified Aβ peptides (AβpE3) (pGlu)(Probiodrug). Images of sections labelled with each of the antibodies can be viewed with the filmstrip viewer link. The atlas overlayed sections are available using the LocaliZoom tool as outlined above.
One tg2576 female mouse (m-543;18 months) was labelled by triple immunofluorescence labelling with antibodies against AβpE3 (J8) conj. with Cy2 (green); α-synuclein (C20R) conj. with Cy3 (orange); Aβ peptide (aa 17-24) (4G8) conjugated with Cy5 (red) and Hoest labelling of Nuclei (Dapi channel)(blue). The triple labelling shows localization of α-synuclein close to amyloid plaques, thereby comforting the CrossSeeding hypothesis.
Human APP (1D1)
Mouse Mtg01_bl1 (Female): filmstrip Localizoom (Ctrl+click to open in a new window)
Mouse m-287 (Male): filmstrip Localizoom
Aβ peptide (aa 17-24)(4G8)
Mouse Mtg01_bl1 (Female): filmstrip Localizoom
Mouse m-287 (Male): filmstrip Localizoom
Aβ peptide (aa 1-4) MOAB-2
Mouse Mtg01_bl1 (Female): filmstrip Localizoom
Mouse m-287 (Male): filmstrip Localizoom
AβpE3 (pGlu)
Mouse Mtg01_bl1 (Female): filmstrip Localizoom
Mouse m-287 (Male): filmstrip Localizoom
Triple labelling (4G8; C20R and pGlu)
Mouse m-543_bl1 (Female): filmstrip
AD rat (McGill-R-Thy1-APP)
This 16 month old male rat was labelled with with 4 different antibodies: against human APP (1D1) (Höfling et al. 2016 Aging Cell), against Aβ peptide aa 17-24 (BioLegend,Cat:4G8), against Aβ peptide (aa 1-4) but not APP (Cayman Chemicals, Cat:MOAB-2) and against Pyroglutamate-modified Aβ peptides (AβpE3) (pGlu)(Probiodrug). Images of sections labelled with each of the antibodies can be viewed with the filmstrip viewer link. The atlas overlayed sections are available using the LocaliZoom tool as outlined above.
Human APP (1D1)
Rat r4753 (Male): filmstrip Localizoom
Aβ peptide (aa 17-24)(4G8)
Rat r4753 (Male): filmstrip Localizoom
Aβ peptide (aa 1-4) MOAB-2
Rat r4753 (Male): filmstrip Localizoom
AβpE3 (pGlu)
Rat r4753 (Male): filmstrip Localizoom
PD mouse (TetO-A53T-PITX3)
A 6 month old mouse was labelled with 3 different antibodies: against human α-synuclein (aa 117-131) (BML-SA3400) (Enzo Lifesciences), against mouse α-synuclein (D37A6) (Cell Signalling) and against Phospho-α-synuclein (Ser129) (EP1536Y)(Abcam, Cat:ab51253).
Human α-synuclein (aa 117-131)
Mouse M9 : filmstrip Localizoom
Mouse α-synuclein (D37A6)
Mouse M9 : filmstrip Localizoom
Phospho-α-synuclein (Ser129)
Mouse M9 : filmstrip Localizoom
PD rat (BAC-SNCA)
Two transgenic male Parkinson rats (Tg5573; 15 month oldand Tg 5141; 12 months old) and two wild type rats (WT 5518 and WT5559;15 months old) were labelled with antibodies against α-synuclein: against α-synuclein Syn1 (endogenous) (BD, Cat: 610787), against aggregated α-synuclein (Millipore, Cat.: MABN389), against alpha Synuclein 15G7 (human) (Enzo, Cat.: ALX-804-258). In addition, these animals were labelled with antibodies against Aβ peptide aa 17-24 (BioLegend, Cat:4G8), against pyroGlu-alpha Synuclein (pGlu79) (Probiodrug) and against Huntingtin (Epitomics/Abcam, Cat: ab45169).
Syn1 (endogenous)
Tg5573 (Male) : filmstrip Localizoom
WT5518 (Male): filmstrip Localizoom
MABN389 (aggregated)
Tg5573 (Male) : filmstrip Localizoom
WT5518 (Male): filmstrip Localizoom
15G7 (human)
Tg5573 (Male) : filmstrip Localizoom
WT5518 (Male): filmstrip Localizoom
Aβ peptide (aa 17-24)(4G8)
Tg5573 (Male) : filmstrip Localizoom
WT5518 (Male): filmstrip Localizoom
pGlu79 α-synuclein
Tg5573 (Male) : filmstrip Localizoom
WT5518 (Male): filmstrip Localizoom
Huntingtin
Tg3876 (Male): filmstrip Localizoom
WT5559 (Male): filmstrip Localizoom
HD F344tg rat
Two transgenic female Huntington rats ( Tg3898 and Tg3876; 90 weeks) and two female Wild type Fisher rats (WT 3949 and WT3884; 90weeks old) were labelled with antibodies against Huntingtin: against 2B7 antibody (mouse monoclonal, recognizes the N-terminus of Htt. CHDI foundation); against 3B5H10 antibody against Human Huntingtin (N-terminal 1-171 amino acid fragment of human Huntingtin with a 68 glutamine stretch) (Absolute antibody, Cat: A00144-1.1); against S829 (recognize mutated Htt aggregates, exon 1 (53Q)hHtt; gift from Prof. G. Bates).
3B5H10
- 766_2724_3446_tg3898_s049 Localizoom
- 766_2724_3446_tg3898_s157 Localizoom
- 766_2724_3446_tg3898_s265 Localizoom
