Difference between revisions of "Tg-Arc-Swe Atlas"

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== About ==
 
== About ==
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The tg-ArcSwe atlas provides access to Aβx-40 immunolabeled histological section images from representative 12 month old tg-ArcSwe mice, showing the morphology and spatial distribution of Aβx-40 plaque deposits across the brain.
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Transgenic mice carrying the Arctic (E693G) and Swedish (KM670/6701NL) amyloid-b precursor protein (AβPP) develop amyloid-beta (Aβ) deposits in the brain that resemble Alzheimer’s disease neuropathology. The tg-ArcSwe atlas provides access to Aβx-40 immunolabeled histological section images from representative 12 month old tg-ArcSwe mice (Lord et al., Neurobiol. Aging 27:67-77, 2006), showing the morphology and spatial distribution of Aβx-40 plaque deposits across the brain.
  
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The Virtual Microscope tool allows users to navigate within and across sagittal section images.  
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== Access image repository ==
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The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link.
  
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Re-use of data from this repository is allowed provided that reference is given to the following publication: Lillehaug S, Syverstad GH, Nilsson LNG,Bjaalie JG, Leergaard TB, Torp R (2014): Brainwide distribution and variance of amyloid-beta deposits in tg-ArcSwe mice. Neurobiology og Aging, 35:556-564 (doi: 10.1016/j.neurobiolaging.2013.09.013).
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'''Re-use of data from this repository is allowed provided that reference is given to the following publication:''' Lillehaug S, Syverstad GH, Nilsson LNG,Bjaalie JG, Leergaard TB, Torp R (2014): Brainwide distribution and variance of amyloid-beta deposits in tg-ArcSwe mice. Neurobiology of Aging, 35:556-564 (doi: 10.1016/j.neurobiolaging.2013.09.013).
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{| class="wikitable"
 +
!Case #
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!Animal
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!Image repository
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!Download
 +
|-
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|F3
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|Female tg-ArcSwe, 12 months
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|[http://cmbn-navigator.uio.no/navigator/filmstrip/index.html?blockId=381 Filmstrip viewer (32 images)]
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|[http://cmbn-navigator.uio.no/navigator/feeder/all_originals/?id=381 Tiffs]
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|-
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|M3
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|Male tg-ArcSwe, 12 months
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|[http://cmbn-navigator.uio.no/navigator/filmstrip/index.html?blockId=403 Filmstrip viewer (17 images)]
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|[http://cmbn-navigator.uio.no/navigator/feeder/all_originals/?id=403 Tiffs]
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|}
  
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Female tg-ArcSwe, 12 months
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Supporting material
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(3x images, case F3)
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{| class="wikitable"
 +
!Case #
 +
!Animal
 +
!Image repository
 +
!Download
 +
|-
 +
|F2
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|Female tg-ArcSwe, 12 months
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|[http://cmbn-navigator.uio.no/navigator/filmstrip/index.html?blockId=427 Filmstrip viewer (4 images)]
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|[http://cmbn-navigator.uio.no/navigator/feeder/all_originals/?id=427 Tiffs]
 +
|-
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|M4
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|Male tg-ArcSwe, 12 months
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|[http://cmbn-navigator.uio.no/navigator/filmstrip/index.html?blockId=424 Filmstrip viewer (4 images)]
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|[http://cmbn-navigator.uio.no/navigator/feeder/all_originals/?id=424 Tiffs]
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|}
  
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Male tg-ArcSwe, 12 months
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== Experimental procedures in brief ==
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(17 images, case M3)
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* Animals: mice carrying a human APP cDNA with the Arctic (p. E693G) and Swedish (p. KM670/671NL) mutations
  
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Additional images
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* Immunohistochemistry:
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** Sagittal sections from the right hemisphere were cryosectioned at 25 µm.
 +
** Amyloid deposits were identified using primary antibodies Aβx-40, visualized using streptavidin-biotinylated horseradish peroxidase comples and diaminobenzidine tetrahydrochloride
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** Digital images were created using a slidescanner (Mirax Scan, Carl Zeiss Microimaging, Jena, Germany)
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For further details, see Lillehaug et al., Neurobiol Aging 35:556-564, 2013.
  
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Female tg-ArcSwe, 12 months
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== Contributing laboratories ==
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(2 images, case F1)
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'''Laboratory of Molecular Neuroscience, '''Institute of Basic Medical Sciences - Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway. Animal breeding, immunohistochemistry. '''People''': Gry H. Syverstad, Reidun Torp
  
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Relatively loose plaque morphology
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'''Department of Pharmacology''', Institute of Clinical Medicine, University of Oslo & Oslo University Hospital, P.O. Box 1057 Blindern, N - 0316 Oslo, Norway. Animal breeding. '''People:''' Lars N.G. Nilsson
  
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Male tg-ArcSwe, 12 months
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'''Neural Systems Laboratory''' (http://www.nesys.uio.no), Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Image acquisition, Histochemistry, Atlas repository. '''People:''' Sveinung Lillehaug, Dmitri Darine, Gergely Csucs, Grazyna Babinska, Trygve B. Leergaard, Jan G. Bjaalie
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(2 images, case M4)
 
  
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Small, dense plaque deposits
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== Funded by: ==
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* The neuroinformatics components of this resource have been funded by the Human Brain Project through the European Union Seventh Framework Program (FP7/2007-2013) under grant agreement no. 604102 (HBP), and The Research Council of Norway (INCF National Node, project 218842).
  
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For details, please cf. Lillehaug, Syverstad et al., (Neurobiology of Aging, 2014, 35:556-564).
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==Contact==
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j.g.bjaalie@medisin.uio.no
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Animals: mice carrying a human APP cDNA with the Arctic (p. E693G) and Swedish (p. KM670/671NL) mutations
 
−
 
 
−
Immunohistochemistry:
 
−
 
 
−
Sagittal sections from right hemisphere were cryosectioned at 25 µm.
 
−
 
 
−
Amyloid deposits were identified using primary antibodies Aβx-40, visualized using streptavidin-biotinylated horseradish peroxidase comples and diaminobenzidine tetrahydrochloride
 
−
 
 
−
Digital images were created using a slidescanner (Mirax Scan, Carl Zeiss Microimaging, Jena, Germany)
 
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Credits
 
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Animal breeding: Reidun Torp, Lars N. G. Nilsson
 
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Immunohistochemistry: Gry H. Syverstad, Reidun Torp, Grazyna Babinska
 
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tg-ArcSwe atlas development team: Sveinung Lillehaug, Dmitri Darine, Gergely Scucs, Jan G. Bjaalie, Trygve B. Leergaard
 
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Sveinung Lillehaug, Jan G. Bjaalie, Trygve B. Leergaard
 
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Neural Systems and Graphics Computing Laboratory 
 
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Institute of Basic Medical Sciences - Anatomy
 
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University of Oslo
 
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P.O. Box 1105 Blindern
 
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N - 0317 Oslo
 
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Norway
 
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http://www.nesys.uio.no
 
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http://www.rbwb.org
 
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Gry Helen Enger Syverstad, Reidun Torp
 
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Laboratory of Molecular Neuroscience
 
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Institute of Basic Medical Sciences - Anatomy
 
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University of Oslo
 
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P.O. Box 1105 Blindern
 
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N - 0317 Oslo
 
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Norway
 
−
 
 
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Lars N.G. Nilsson
 
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Department of Pharmacology, Institute of Clinical Medicine, University of Oslo & Oslo University Hospital
 
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P.O. Box 1057 Blindern
 
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N - 0316 Oslo
 
−
Norway
 

Latest revision as of 15:57, 16 February 2021

About

Transgenic mice carrying the Arctic (E693G) and Swedish (KM670/6701NL) amyloid-b precursor protein (AβPP) develop amyloid-beta (Aβ) deposits in the brain that resemble Alzheimer’s disease neuropathology. The tg-ArcSwe atlas provides access to Aβx-40 immunolabeled histological section images from representative 12 month old tg-ArcSwe mice (Lord et al., Neurobiol. Aging 27:67-77, 2006), showing the morphology and spatial distribution of Aβx-40 plaque deposits across the brain.

Access image repository

The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link.

Re-use of data from this repository is allowed provided that reference is given to the following publication: Lillehaug S, Syverstad GH, Nilsson LNG,Bjaalie JG, Leergaard TB, Torp R (2014): Brainwide distribution and variance of amyloid-beta deposits in tg-ArcSwe mice. Neurobiology of Aging, 35:556-564 (doi: 10.1016/j.neurobiolaging.2013.09.013).

Case # Animal Image repository Download
F3 Female tg-ArcSwe, 12 months Filmstrip viewer (32 images) Tiffs
M3 Male tg-ArcSwe, 12 months Filmstrip viewer (17 images) Tiffs

Supporting material

Case # Animal Image repository Download
F2 Female tg-ArcSwe, 12 months Filmstrip viewer (4 images) Tiffs
M4 Male tg-ArcSwe, 12 months Filmstrip viewer (4 images) Tiffs

Experimental procedures in brief

  • Animals: mice carrying a human APP cDNA with the Arctic (p. E693G) and Swedish (p. KM670/671NL) mutations
  • Immunohistochemistry:
    • Sagittal sections from the right hemisphere were cryosectioned at 25 µm.
    • Amyloid deposits were identified using primary antibodies Aβx-40, visualized using streptavidin-biotinylated horseradish peroxidase comples and diaminobenzidine tetrahydrochloride
    • Digital images were created using a slidescanner (Mirax Scan, Carl Zeiss Microimaging, Jena, Germany)

For further details, see Lillehaug et al., Neurobiol Aging 35:556-564, 2013.

Contributing laboratories

Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences - Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway. Animal breeding, immunohistochemistry. People: Gry H. Syverstad, Reidun Torp

Department of Pharmacology, Institute of Clinical Medicine, University of Oslo & Oslo University Hospital, P.O. Box 1057 Blindern, N - 0316 Oslo, Norway. Animal breeding. People: Lars N.G. Nilsson

Neural Systems Laboratory (http://www.nesys.uio.no), Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Image acquisition, Histochemistry, Atlas repository. People: Sveinung Lillehaug, Dmitri Darine, Gergely Csucs, Grazyna Babinska, Trygve B. Leergaard, Jan G. Bjaalie

Funded by:

  • The neuroinformatics components of this resource have been funded by the Human Brain Project through the European Union Seventh Framework Program (FP7/2007-2013) under grant agreement no. 604102 (HBP), and The Research Council of Norway (INCF National Node, project 218842).

Contact

j.g.bjaalie@medisin.uio.no

Retrieved from "https://wiki.uio.no/projects/navigator3/index.php?title=Tg-Arc-Swe_Atlas&oldid=954"