Difference between revisions of "Tg-Arc-Swe Atlas"
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The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link. | The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link. | ||
| − | '''Re-use of data from this repository is allowed provided that reference is given to the following publication:''' Lillehaug S, Syverstad GH, Nilsson LNG,Bjaalie JG, Leergaard TB, Torp R (2014): Brainwide distribution and variance of amyloid-beta deposits in tg-ArcSwe mice. Neurobiology | + | '''Re-use of data from this repository is allowed provided that reference is given to the following publication:''' Lillehaug S, Syverstad GH, Nilsson LNG,Bjaalie JG, Leergaard TB, Torp R (2014): Brainwide distribution and variance of amyloid-beta deposits in tg-ArcSwe mice. Neurobiology of Aging, 35:556-564 (doi: 10.1016/j.neurobiolaging.2013.09.013). |
{| class="wikitable" | {| class="wikitable" | ||
| − | ! | + | !Case # |
| − | ! | + | !Animal |
| − | ! | + | !Image repository |
| − | ! | + | !Download |
|- | |- | ||
| − | | | + | |F3 |
| − | | | + | |Female tg-ArcSwe, 12 months |
| − | | | + | |Filmstrip viewer (32 images) |
| − | | | + | |Tiffs |
|- | |- | ||
| − | | | + | |M3 |
| − | | | + | |Male tg-ArcSwe, 12 months |
| − | | | + | |Filmstrip viewer (17 images) |
| − | | | + | |Tiffs |
| + | |} | ||
| + | |||
| + | Supporting material | ||
| + | {| class="wikitable" | ||
| + | !Case # | ||
| + | !Animal | ||
| + | !Image repository | ||
| + | !Download | ||
|- | |- | ||
| − | | | + | |F1 |
| − | | | + | |Female tg-ArcSwe, 12 months |
| − | | | + | |Filmstrip viewer (2 images) |
| − | | | + | |Tiffs |
| + | |- | ||
| + | |M4 | ||
| + | |Male tg-ArcSwe, 12 months | ||
| + | |Filmstrip viewer (2 images) | ||
| + | |Tiffs | ||
|} | |} | ||
| − | + | == Experimental procedures in brief == | |
| − | + | * Animals: mice carrying a human APP cDNA with the Arctic (p. E693G) and Swedish (p. KM670/671NL) mutations | |
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| − | Animals: mice carrying a human APP cDNA with the Arctic (p. E693G) and Swedish (p. KM670/671NL) mutations | ||
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| − | Immunohistochemistry: | + | * Immunohistochemistry: |
| + | ** Sagittal sections from right hemisphere were cryosectioned at 25 µm. | ||
| + | ** Amyloid deposits were identified using primary antibodies Aβx-40, visualized using streptavidin-biotinylated horseradish peroxidase comples and diaminobenzidine tetrahydrochloride | ||
| + | ** Digital images were created using a slidescanner (Mirax Scan, Carl Zeiss Microimaging, Jena, Germany) | ||
| + | For further details, see Lillehaug et al., Neurobiol Aging 35:556-564, 2013. | ||
| − | + | == Contributing laboratories == | |
| + | '''Laboratory of Molecular Neuroscience, '''Institute of Basic Medical Sciences - Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway. Animal breeding, immunohistochemistry. '''People''': Gry H. Syverstad, Reidun Torp | ||
| − | + | '''Department of Pharmacology''', Institute of Clinical Medicine, University of Oslo & Oslo University Hospital, P.O. Box 1057 Blindern, N - 0316 Oslo, Norway. Animal breeding. '''People:''' Lars N.G. Nilsson | |
| − | |||
| − | Institute of | ||
| − | University of Oslo | ||
| − | P.O. Box | ||
| − | N - | ||
| − | Norway | ||
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| − | + | '''Neural Systems Laboratory''' (http://www.nesys.uio.no), Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Image acquisition, Histochemistry, Atlas repository. '''People:''' Sveinung Lillehaug, Dmitri Darine, Gergely Csucs, Grazyna Babinska, Trygve B. Leergaard, Jan G. Bjaalie | |
| − | |||
| − | Institute of Basic Medical Sciences | ||
| − | University of Oslo | ||
| − | P.O. Box 1105 Blindern | ||
| − | N - 0317 Oslo | ||
| − | Norway | ||
| − | + | == Contact == | |
| − | + | j.g.bjaalie@medisin.uio.no | |
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Revision as of 15:05, 26 October 2015
Contents
About
Transgenic mice carrying the Arctic (E693G) and Swedish (KM670/6701NL) amyloid-b precursor protein (AβPP) develop amyloid-beta (Aβ) deposits in the brain that resemble Alzheimer’s disease neuropathology. The tg-ArcSwe atlas provides access to Aβx-40 immunolabeled histological section images from representative 12 month old tg-ArcSwe mice (Lord et al., Neurobiol. Aging 27:67-77, 2006), showing the morphology and spatial distribution of Aβx-40 plaque deposits across the brain.
Access image repository
The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link.
Re-use of data from this repository is allowed provided that reference is given to the following publication: Lillehaug S, Syverstad GH, Nilsson LNG,Bjaalie JG, Leergaard TB, Torp R (2014): Brainwide distribution and variance of amyloid-beta deposits in tg-ArcSwe mice. Neurobiology of Aging, 35:556-564 (doi: 10.1016/j.neurobiolaging.2013.09.013).
| Case # | Animal | Image repository | Download |
|---|---|---|---|
| F3 | Female tg-ArcSwe, 12 months | Filmstrip viewer (32 images) | Tiffs |
| M3 | Male tg-ArcSwe, 12 months | Filmstrip viewer (17 images) | Tiffs |
Supporting material
| Case # | Animal | Image repository | Download |
|---|---|---|---|
| F1 | Female tg-ArcSwe, 12 months | Filmstrip viewer (2 images) | Tiffs |
| M4 | Male tg-ArcSwe, 12 months | Filmstrip viewer (2 images) | Tiffs |
Experimental procedures in brief
- Animals: mice carrying a human APP cDNA with the Arctic (p. E693G) and Swedish (p. KM670/671NL) mutations
- Immunohistochemistry:
- Sagittal sections from right hemisphere were cryosectioned at 25 µm.
- Amyloid deposits were identified using primary antibodies Aβx-40, visualized using streptavidin-biotinylated horseradish peroxidase comples and diaminobenzidine tetrahydrochloride
- Digital images were created using a slidescanner (Mirax Scan, Carl Zeiss Microimaging, Jena, Germany)
For further details, see Lillehaug et al., Neurobiol Aging 35:556-564, 2013.
Contributing laboratories
Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences - Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway. Animal breeding, immunohistochemistry. People: Gry H. Syverstad, Reidun Torp
Department of Pharmacology, Institute of Clinical Medicine, University of Oslo & Oslo University Hospital, P.O. Box 1057 Blindern, N - 0316 Oslo, Norway. Animal breeding. People: Lars N.G. Nilsson
Neural Systems Laboratory (http://www.nesys.uio.no), Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Image acquisition, Histochemistry, Atlas repository. People: Sveinung Lillehaug, Dmitri Darine, Gergely Csucs, Grazyna Babinska, Trygve B. Leergaard, Jan G. Bjaalie
Contact
j.g.bjaalie@medisin.uio.no
