Difference between revisions of "Tg-Arc-Swe Atlas"
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* Immunohistochemistry: | * Immunohistochemistry: | ||
− | ** Sagittal sections from right hemisphere were cryosectioned at 25 µm. | + | ** Sagittal sections from the right hemisphere were cryosectioned at 25 µm. |
** Amyloid deposits were identified using primary antibodies Aβx-40, visualized using streptavidin-biotinylated horseradish peroxidase comples and diaminobenzidine tetrahydrochloride | ** Amyloid deposits were identified using primary antibodies Aβx-40, visualized using streptavidin-biotinylated horseradish peroxidase comples and diaminobenzidine tetrahydrochloride | ||
** Digital images were created using a slidescanner (Mirax Scan, Carl Zeiss Microimaging, Jena, Germany) | ** Digital images were created using a slidescanner (Mirax Scan, Carl Zeiss Microimaging, Jena, Germany) |
Revision as of 21:55, 4 December 2015
Contents
About
Transgenic mice carrying the Arctic (E693G) and Swedish (KM670/6701NL) amyloid-b precursor protein (AβPP) develop amyloid-beta (Aβ) deposits in the brain that resemble Alzheimer’s disease neuropathology. The tg-ArcSwe atlas provides access to Aβx-40 immunolabeled histological section images from representative 12 month old tg-ArcSwe mice (Lord et al., Neurobiol. Aging 27:67-77, 2006), showing the morphology and spatial distribution of Aβx-40 plaque deposits across the brain.
Access image repository
The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link.
Re-use of data from this repository is allowed provided that reference is given to the following publication: Lillehaug S, Syverstad GH, Nilsson LNG,Bjaalie JG, Leergaard TB, Torp R (2014): Brainwide distribution and variance of amyloid-beta deposits in tg-ArcSwe mice. Neurobiology of Aging, 35:556-564 (doi: 10.1016/j.neurobiolaging.2013.09.013).
Case # | Animal | Image repository | Download |
---|---|---|---|
F3 | Female tg-ArcSwe, 12 months | Filmstrip viewer (32 images) | Tiffs |
M3 | Male tg-ArcSwe, 12 months | Filmstrip viewer (17 images) | Tiffs |
Supporting material
Case # | Animal | Image repository | Download |
---|---|---|---|
F2 | Female tg-ArcSwe, 12 months | Filmstrip viewer (2 images) | Tiffs |
M4 | Male tg-ArcSwe, 12 months | Filmstrip viewer (2 images) | Tiffs |
Experimental procedures in brief
- Animals: mice carrying a human APP cDNA with the Arctic (p. E693G) and Swedish (p. KM670/671NL) mutations
- Immunohistochemistry:
- Sagittal sections from the right hemisphere were cryosectioned at 25 µm.
- Amyloid deposits were identified using primary antibodies Aβx-40, visualized using streptavidin-biotinylated horseradish peroxidase comples and diaminobenzidine tetrahydrochloride
- Digital images were created using a slidescanner (Mirax Scan, Carl Zeiss Microimaging, Jena, Germany)
For further details, see Lillehaug et al., Neurobiol Aging 35:556-564, 2013.
Contributing laboratories
Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences - Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway. Animal breeding, immunohistochemistry. People: Gry H. Syverstad, Reidun Torp
Department of Pharmacology, Institute of Clinical Medicine, University of Oslo & Oslo University Hospital, P.O. Box 1057 Blindern, N - 0316 Oslo, Norway. Animal breeding. People: Lars N.G. Nilsson
Neural Systems Laboratory (http://www.nesys.uio.no), Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Image acquisition, Histochemistry, Atlas repository. People: Sveinung Lillehaug, Dmitri Darine, Gergely Csucs, Grazyna Babinska, Trygve B. Leergaard, Jan G. Bjaalie
Contact
j.g.bjaalie@medisin.uio.no