Difference between revisions of "Tg-Arc-Swe Atlas"

From projects.navigator3
Jump to: navigation, search
(Created page with "About The tg-ArcSwe atlas provides access to Aβx-40 immunolabeled histological section images from representative 12 month old tg-ArcSwe mice, showing the morphology and spat...")
 
Line 1: Line 1:
−
About
+
== About ==
 
The tg-ArcSwe atlas provides access to Aβx-40 immunolabeled histological section images from representative 12 month old tg-ArcSwe mice, showing the morphology and spatial distribution of Aβx-40 plaque deposits across the brain.
 
The tg-ArcSwe atlas provides access to Aβx-40 immunolabeled histological section images from representative 12 month old tg-ArcSwe mice, showing the morphology and spatial distribution of Aβx-40 plaque deposits across the brain.
 +
 +
The Virtual Microscope tool allows users to navigate within and across sagittal section images.
 +
 +
Re-use of data from this repository is allowed provided that reference is given to the following publication: Lillehaug S, Syverstad GH, Nilsson LNG,Bjaalie JG, Leergaard TB, Torp R (2014): Brainwide distribution and variance of amyloid-beta deposits in tg-ArcSwe mice. Neurobiology og Aging, 35:556-564 (doi: 10.1016/j.neurobiolaging.2013.09.013).
 +
 +
Female tg-ArcSwe, 12 months
 +
(3x images, case F3)
 +
 +
Male tg-ArcSwe, 12 months
 +
(17 images, case M3)
 +
 +
Additional images
 +
 +
Female tg-ArcSwe, 12 months
 +
(2 images, case F1)
 +
 +
Relatively loose plaque morphology
 +
 +
Male tg-ArcSwe, 12 months
 +
(2 images, case M4)
 +
 +
Small, dense plaque deposits
 +
 +
For details, please cf. Lillehaug, Syverstad et al., (Neurobiology of Aging, 2014, 35:556-564).
 +
 +
Animals: mice carrying a human APP cDNA with the Arctic (p. E693G) and Swedish (p. KM670/671NL) mutations
 +
 +
Immunohistochemistry:
 +
 +
Sagittal sections from right hemisphere were cryosectioned at 25 µm.
 +
 +
Amyloid deposits were identified using primary antibodies Aβx-40, visualized using streptavidin-biotinylated horseradish peroxidase comples and diaminobenzidine tetrahydrochloride
 +
 +
Digital images were created using a slidescanner (Mirax Scan, Carl Zeiss Microimaging, Jena, Germany)
 +
 +
Credits
 +
 +
Animal breeding: Reidun Torp, Lars N. G. Nilsson
 +
 +
Immunohistochemistry: Gry H. Syverstad, Reidun Torp, Grazyna Babinska
 +
 +
tg-ArcSwe atlas development team: Sveinung Lillehaug, Dmitri Darine, Gergely Scucs, Jan G. Bjaalie, Trygve B. Leergaard
 +
 +
Sveinung Lillehaug, Jan G. Bjaalie, Trygve B. Leergaard
 +
Neural Systems and Graphics Computing Laboratory 
 +
Institute of Basic Medical Sciences - Anatomy
 +
University of Oslo
 +
P.O. Box 1105 Blindern
 +
N - 0317 Oslo
 +
Norway
 +
http://www.nesys.uio.no
 +
http://www.rbwb.org
 +
 +
Gry Helen Enger Syverstad, Reidun Torp
 +
Laboratory of Molecular Neuroscience
 +
Institute of Basic Medical Sciences - Anatomy
 +
University of Oslo
 +
P.O. Box 1105 Blindern
 +
N - 0317 Oslo
 +
Norway
 +
 +
Lars N.G. Nilsson
 +
Department of Pharmacology, Institute of Clinical Medicine, University of Oslo & Oslo University Hospital
 +
P.O. Box 1057 Blindern
 +
N - 0316 Oslo
 +
Norway

Revision as of 14:42, 26 October 2015

About

The tg-ArcSwe atlas provides access to Aβx-40 immunolabeled histological section images from representative 12 month old tg-ArcSwe mice, showing the morphology and spatial distribution of Aβx-40 plaque deposits across the brain.

The Virtual Microscope tool allows users to navigate within and across sagittal section images.

Re-use of data from this repository is allowed provided that reference is given to the following publication: Lillehaug S, Syverstad GH, Nilsson LNG,Bjaalie JG, Leergaard TB, Torp R (2014): Brainwide distribution and variance of amyloid-beta deposits in tg-ArcSwe mice. Neurobiology og Aging, 35:556-564 (doi: 10.1016/j.neurobiolaging.2013.09.013).

Female tg-ArcSwe, 12 months (3x images, case F3)

Male tg-ArcSwe, 12 months (17 images, case M3)

Additional images

Female tg-ArcSwe, 12 months (2 images, case F1)

Relatively loose plaque morphology

Male tg-ArcSwe, 12 months (2 images, case M4)

Small, dense plaque deposits

For details, please cf. Lillehaug, Syverstad et al., (Neurobiology of Aging, 2014, 35:556-564).

Animals: mice carrying a human APP cDNA with the Arctic (p. E693G) and Swedish (p. KM670/671NL) mutations

Immunohistochemistry:

Sagittal sections from right hemisphere were cryosectioned at 25 µm.

Amyloid deposits were identified using primary antibodies Aβx-40, visualized using streptavidin-biotinylated horseradish peroxidase comples and diaminobenzidine tetrahydrochloride

Digital images were created using a slidescanner (Mirax Scan, Carl Zeiss Microimaging, Jena, Germany)

Credits

Animal breeding: Reidun Torp, Lars N. G. Nilsson

Immunohistochemistry: Gry H. Syverstad, Reidun Torp, Grazyna Babinska

tg-ArcSwe atlas development team: Sveinung Lillehaug, Dmitri Darine, Gergely Scucs, Jan G. Bjaalie, Trygve B. Leergaard

Sveinung Lillehaug, Jan G. Bjaalie, Trygve B. Leergaard Neural Systems and Graphics Computing Laboratory  Institute of Basic Medical Sciences - Anatomy University of Oslo P.O. Box 1105 Blindern N - 0317 Oslo Norway http://www.nesys.uio.no http://www.rbwb.org

Gry Helen Enger Syverstad, Reidun Torp Laboratory of Molecular Neuroscience Institute of Basic Medical Sciences - Anatomy University of Oslo P.O. Box 1105 Blindern N - 0317 Oslo Norway

Lars N.G. Nilsson Department of Pharmacology, Institute of Clinical Medicine, University of Oslo & Oslo University Hospital P.O. Box 1057 Blindern N - 0316 Oslo Norway

Retrieved from "https://wiki.uio.no/projects/navigator3/index.php?title=Tg-Arc-Swe_Atlas&oldid=736"