TTA brain Atlas

TTA / TetOff Atlas

Insert non-formatted text hereAbout[edit | edit source] The tTA atlas III (Nop) is an interactive resource providing access to brain-wide histological images documenting the activity of the Nop-tTA promoter in bigenic mice in which the Nop-tTA promoter regulates the expression of the LacZ reporter gene, encoding the histologically detectible marker β-galactosidase. Detailed knowledge about where in the brain gene expression is regulated is essential for the use and interpretation of such models. Access image repository[edit | edit source] The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link.Re-use of data from this repository is allowed provided that reference is given to the following publication: Yetman MJ, Lillehaug S, Bjaalie JG, Leergaard TB, Jankowsky JL. Transgene expression in the Nop-TTA driver line is not inherently restricted to the entorhinal cortex. Brain Struct Funct. 2015. DOI 10.1007/s00429-015-1040-9 Animal # Genotype Section orientation Image repository Download images 2849 Nop-tTA/tetO-lacZ-nls-GFP Coronal Filmstrip viewer Tiffs 1952 Nop-tTA/tetO-lacZ-nls-GFP Coronal Filmstrip viewer Tiffs 2877 Nop-tTA/tetO-lacZ-nls-GFP Horizontal Filmstrip viewer Tiffs 1950 tetO-lacZ-nls-GFP (control) Coronal Filmstrip viewer Tiffs Experimental procedures in brief[edit | edit source] The atlas is based on data from adult 4-6 month old bigenic Nop-tTA/tetO-lacZ-nls-GFP mice and adult 4-6 month old single transgenic tetO-lacZ-nls-GFP mice (controls). Sagittal sections from right hemisphere were cryosectioned at 40 µm. β-galactosidase was labeled blue using X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) as a substrate. Weak counterstaining with Neutral Nuclear Fast Red facilitated detection of boundaries and landmarks without compromising the observation of X-gal labeling. Digital images were created using a slidescanner (AxioScan Z1, Carl Zeiss Microimaging, Jena, Germany).