CrossSeeds
Contents
About
The CrossSeeds project hypothesis is that AD, PD and HD share common pathways of protein aggregation and that cross-seeding of pathogenic pGlu-modified proteins are leading to neurodegeneration. This site demonstrates ongoing efforts in mapping of the distribution of pGlu-protein expression sites in rat and mouse models. High resolution histological section images from whole brains are organized in a database and anchored to 3D reference atlas space (Waxholm rat brain atlas and Allen mouse brain mouse atlas). The data system in preparation allows storage of all raw data, data retrieval, and visualization and analysis in 3D space.
Image repository, viewing, and analysis
The virtual microscopy filmstrip viewer allows interactive zooming and panning of images.
The Localizoom viewer uses the atlas anchoring information and allows visualization of single section images with a transparent atlas-segmentation overlay, matching the location in the brain of the section image and cut at the custom angle of orientation of the histological section. Names of atlas structures are shown when pointing the mouse over the region of interest and 3D coordinates at the location of the cursor are shown real-time. An extended version of the LocaliZoom can harvest coordinates corresponding to selected features of interest in the images for further 3D analysis. Zooming in a specific area is possible using the mouse wheel. Transparency of the atlas is adjustable on the top left corner scale.
AD mouse tg2576
This 18 month old Alzheimer’s mouse was labelled with 4 different antibodies against human APP (1D1), against APP and Abeta (4G8), against Abeta but not APP (MOAB-2) and against pGlu-Abeta. Images of example sections labelled with each of the antibodies can be viewed with the filmstrip viewer and the LocaliZoom tool as outlined above.
1D1
- 761_2222_2904_Mtg01_bl1_1D1_s014 Localizoom
- 761_2222_2904_Mtg01_bl1_1D1_s028 Localizoom
- 761_2222_2904_Mtg01_bl1_1D1_s041 Localizoom
MOAB2
- 761_2222_2942_Mtg01_bl1_MOAB2_s015 Localizoom
- 761_2222_2942_Mtg01_bl1_MOAB2_s091 Localizoom
- 761_2222_2942_Mtg01_bl1_MOAB2_s143 Localizoom
pGlu
- 761_2222_2943_Mtg01_bl1_pGlu_s032 Localizoom
- 761_2222_2943_Mtg01_bl1_pGlu_s092 Localizoom
- 761_2222_2943_Mtg01_bl1_pGlu_s144 Localizoom
4G8
- 761_2222_2941_Mtg01_bl1_4G8_s026 Localizoom
- 761_2222_2941_Mtg01_bl1_4G8_s090 Localizoom
- 761_2222_2941_Mtg01_bl1_4G8_s154 Localizoom
HD F344tg rat
This 20 month old rat was labelled with the 3B5H10 antibody against Human Huntingtin. Images of example sections can be viewed with the filmstrip viewer and the LocaliZoom tool as outlined above.
3B5H10
- 766_2724_3446_tg3898_s049 Localizoom
- 766_2724_3446_tg3898_s157 Localizoom
- 766_2724_3446_tg3898_s265 Localizoom
