CrossSeeds
Contents
About
The CrossSeeds project hypothesis is that AD, PD and HD share common pathways of protein aggregation and that cross-seeding of pathogenic pGlu-modified proteins are leading to neurodegeneration. This site demonstrates ongoing efforts in mapping of the distribution of pGlu-protein expression sites in rat and mouse models. High resolution histological section images from whole brains are organized in a database and anchored to 3D reference atlas space (Waxholm rat brain atlas and Allen mouse brain mouse atlas). The data system in preparation allows storage of all raw data, data retrieval, and visualization and analysis in 3D space.
Image repository, viewing, and analysis
The virtual microscopy filmstrip viewer allows interactive zooming and panning of images.
The Localizoom viewer uses the atlas anchoring information and allows visualization of single section images with a transparent atlas-segmentation overlay, matching the location in the brain of the section image and cut at the custom angle of orientation of the histological section. Names of atlas structures are shown when pointing the mouse over the region of interest and 3D coordinates at the location of the cursor are shown real-time. An extended version of the LocaliZoom can harvest coordinates corresponding to selected features of interest in the images for further 3D analysis. Zooming in a specific area is possible using the mouse wheel. Transparency of the atlas is adjustable on the top left corner scale.
AD mouse tg2576
Two 18 month old Alzheimer’s mice were labelled with 4 different antibodies: against human APP (1D1) (Höfling et al. 2016 Aging Cell), against Aβ peptide aa 17-24 (4G8) (BioLegend), against Aβ peptide (aa 1-4) but not APP (MOAB-2) (Cayman Chemicals) and against Pyroglutamate-modified Aβ peptides (AβpE3) (pGlu)(Probiodrug). Images of sections labelled with each of the antibodies can be viewed with the filmstrip viewer link. The atlas overlayed sections are available using the LocaliZoom tool as outlined above.
Human APP (1D1)
Mouse Mtg01_bl1: filmstrip Localizoom
Mouse m-287: filmstrip Localizoom
Aβ peptide (aa 17-24)(4G8)
Mouse Mtg01_bl1: filmstrip Localizoom
Mouse m-287: filmstrip Localizoom
Aβ peptide (aa 1-4) MOAB-2
Mouse Mtg01_bl1: filmstrip Localizoom
Mouse m-287: filmstrip Localizoom
AβpE3 (pGlu)
Mouse Mtg01_bl1: filmstrip Localizoom
Mouse m-287: filmstrip Localizoom
HD F344tg rat
This 20 month old rat was labelled with the 3B5H10 antibody against Human Huntingtin. Images of example sections can be viewed with the filmstrip viewer and the LocaliZoom tool as outlined above.
3B5H10
- 766_2724_3446_tg3898_s049 Localizoom
- 766_2724_3446_tg3898_s157 Localizoom
- 766_2724_3446_tg3898_s265 Localizoom
