Difference between revisions of "Neurotransporter Atlas: VGLUT3"

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== <span style="font-size:medium"><span style="font-family:georgia,serif"><span style="color: rgb(0, 0, 0); font-weight: bold">VGLUT3 labeling as visible in high-magnification images</span></span></span> ==
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== <span style="font-size:medium"><span style="font-family:georgia,serif"><span style="color: rgb(0, 0, 0); font-weight: bold">VGLUT3 labeling as visible in high-magnification images</span></span></span> ==
  
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| [[File:FigureA VGLUT3.png|thumb|left|500px|A: Punctuate dark-brown labeling representing VGLUT3-positive nerve endings in the pyramidal layer of the hippocampus. Scale bar, 50 µm.]]<br/>
 
| [[File:FigureA VGLUT3.png|thumb|left|500px|A: Punctuate dark-brown labeling representing VGLUT3-positive nerve endings in the pyramidal layer of the hippocampus. Scale bar, 50 µm.]]<br/>
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== <span style="font-size:smaller"><span style="font-family:georgia,serif"><span style="color: rgb(0, 0, 0); font-weight: bold">Experimental procedures</span></span></span> ==
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== <span style="font-size:smaller"><span style="font-family:georgia,serif"><span style="color: rgb(0, 0, 0); font-weight: bold">Experimental procedures</span></span></span> ==
  
 
<span style="font-family:georgia,serif">The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).</span>
 
<span style="font-family:georgia,serif">The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).</span>
  
== <span style="font-size:smaller"><span style="font-family:georgia,serif"><span style="color: rgb(0, 0, 0); font-weight: bold">Atlas coordinate values</span></span></span> ==
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== <span style="font-size:smaller"><span style="font-family:georgia,serif"><span style="color: rgb(0, 0, 0); font-weight: bold">Atlas coordinate values</span></span></span> ==
  
 
<span style="font-family:georgia,serif">Positional coordinates representing distance from Bregma are indicated for each image in the repository. Values were estimated by matching by evaluation of multiple anatomical landmarks in comparison to a standard rat brain atlas (Paxinos and Watson, The Rat Brain in Stereotaxic Coordinates, Elsevier Academic Press, 2007).</span>
 
<span style="font-family:georgia,serif">Positional coordinates representing distance from Bregma are indicated for each image in the repository. Values were estimated by matching by evaluation of multiple anatomical landmarks in comparison to a standard rat brain atlas (Paxinos and Watson, The Rat Brain in Stereotaxic Coordinates, Elsevier Academic Press, 2007).</span>
  
== <span style="font-size:smaller"><span style="font-family:georgia,serif"><span style="color: rgb(0, 0, 0); font-weight: bold">Data sharing</span></span></span> ==
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== <span style="font-size:smaller"><span style="font-family:georgia,serif"><span style="color: rgb(0, 0, 0); font-weight: bold">Data sharing</span></span></span> ==
  
 
<span style="font-family:georgia,serif">Re-use of data from this repository is permitted provided reference is made to the following publication: Stensrud et al. J Comp Neurol, 521:3042-56, 2013. doi: 10.1002/cne.23331.</span>
 
<span style="font-family:georgia,serif">Re-use of data from this repository is permitted provided reference is made to the following publication: Stensrud et al. J Comp Neurol, 521:3042-56, 2013. doi: 10.1002/cne.23331.</span>
  
== <span style="font-size:smaller"><span style="font-family:georgia,serif"><span style="color: rgb(0, 0, 0); font-weight: bold">Access repository and Virtual Microscope</span></span></span> ==
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== <span style="font-size:smaller"><span style="font-family:georgia,serif"><span style="color: rgb(0, 0, 0); font-weight: bold">Access repository and Virtual Microscope</span></span></span> ==
  
 
Access [http://cmbn-navigator.uio.no/neurotransporter_atlas_vglut3/neurotransporter_atlas_vglut3_gui.html Navigator] to explore the data set.
 
Access [http://cmbn-navigator.uio.no/neurotransporter_atlas_vglut3/neurotransporter_atlas_vglut3_gui.html Navigator] to explore the data set.

Revision as of 15:30, 16 February 2015

The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain. The present Virtual Microscope viewing tool allows the user to navigate within and across sagittal section images. The atlas is based on data published by Stensrud et al. 2013 (J Comp Neurol, 521:3042-56).


VGLUT3 labeling as visible in high-magnification images

A: Punctuate dark-brown labeling representing VGLUT3-positive nerve endings in the pyramidal layer of the hippocampus. Scale bar, 50 µm.

B: VGLUT3 stained neuronal cell bodies (arrows) and processes (arrowheads) in the striatum. Scale bar, 50 µm.


Experimental procedures

The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).

Atlas coordinate values

Positional coordinates representing distance from Bregma are indicated for each image in the repository. Values were estimated by matching by evaluation of multiple anatomical landmarks in comparison to a standard rat brain atlas (Paxinos and Watson, The Rat Brain in Stereotaxic Coordinates, Elsevier Academic Press, 2007).

Data sharing

Re-use of data from this repository is permitted provided reference is made to the following publication: Stensrud et al. J Comp Neurol, 521:3042-56, 2013. doi: 10.1002/cne.23331.

Access repository and Virtual Microscope

Access Navigator to explore the data set.

Contributing laboratories

The Neuro- and Gliotransmitter Group - Vidar Gundersen

Centre for Molecular Biology and Neuroscience &
Institute of Basic Medical Sciences - Anatomy
University of Oslo
P.O. Box 1105 Blindern
N - 0317 Oslo
Norway
http://www.med.uio.no/imb/english/research/groups/synaptic-neurochemistry/gliotransmitters/index.html
http://www.cmbn.no


Neural Systems and Graphics Computing Laboratory - Trygve B. Leergaard, Jan G. Bjaalie
Centre for Molecular Biology and Neuroscience &
Institute of Basic Medical Sciences - Anatomy
University of Oslo
P.O. Box 1105 Blindern
N - 0317 Oslo
Norway
http://www.nesys.uio.no
http://www.rbwb.org
http://www.cmbn.no


Credits

Immunohistochemistry

Grazyna Babinska
Mats Julius Stensrud
Vidar Gundersen

NT-atlas web-application development team

Dmitri Darine
Gergely Csucs
Trygve B. Leergaard
Jan G. Bjaalie

Requirements

Web browser requirement: