Difference between revisions of "Neurotransporter Atlas: VGLUT3"

From projects.navigator3
Jump to: navigation, search
(47 intermediate revisions by 4 users not shown)
Line 1: Line 1:
<span style="font-family:georgia,serif;"><span style="color: rgb(0, 0, 0);">The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain. The present Virtual Microscope viewing tool allows the user to navigate within and across sagittal section images. The atlas is based on data published by Stensrud et al. 2013 (J Comp Neurol, 521:3042-56).</span></span>
+
== <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="color: rgb(0, 0, 0); font-weight: bold">About</span></span></span> ==
 +
[[File:FigureA.png|200px|thumb|right|A: Punctuate dark-brown labeling representing VGLUT3-positive nerve endings in the pyramidal layer of the hippocampus. Scale bar, 50 µm.]]
  
 +
[[File:FigureB.png|200px|thumb|right|B: VGLUT3 stained neuronal cell bodies (arrows) and processes (arrowheads) in the striatum. Scale bar, 50 µm.]]
  
 +
Vesicular glutamate transporters (VGLUT1-3) carry glutamate into synaptic vesicles. The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain, visualized by immunohistochemistry using antibodies against VGLUT3.
  
== <span style="font-size:medium;"><span style="font-family:georgia,serif;"><span style="color: rgb(0, 0, 0); font-weight: bold;">VGLUT3 labeling as visible in high-magnification images</span></span></span> ==
+
== <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="color: rgb(0, 0, 0); font-weight: bold">Access image repository</span></span></span> ==
 +
The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link.
  
<gallery widths="260px">
+
'''Re-use of data from this repository is allowed provided that reference is given to the following publication:''' Stensrud MJ, Chaudhry FA, Leergaard TB, Bjaalie JG, Gundersen V. Vesicular glutamate transporter-3 in the rodent brain: vesicular colocalization with vesicular γ-aminobutyric acid transporter. J Comp Neurol (2013) 521:3042–56. doi:10.1002/cne.23331
File:FigureA VGLUT3.png|A: Punctuate dark-brown labeling representing VGLUT3-positive nerve endings in the pyramidal layer of the hippocampus. Scale bar, 50 µm.
+
{| class="wikitable"
File:FigureB VGLUT3.png|B: VGLUT3 stained neuronal cell bodies (arrows) and processes (arrowheads) in the striatum. Scale bar, 50 µm.
+
!Image repository
</gallery>
+
!Download
<div style="clear:both"></div>
+
|-
 +
|[http://cmbn-navigator.uio.no/navigator/filmstrip_viewer.html?publicOnly=true&entityType=block&entityId=222&metadataKeyName=DAB-incubation%20Time# Filmstrip viewer]
 +
|[http://cmbn-navigator.uio.no/navigator/feeder/all_originals/?id=222 Tiffs]
 +
|}
 +
== <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="color: rgb(0, 0, 0); font-weight: bold">Experimental procedures in brief</span></span></span> ==
  
== <span style="font-size:smaller;"><span style="font-family:georgia,serif;"><span style="color: rgb(0, 0, 0); font-weight: bold;">Experimental procedures</span></span></span> ==
+
The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).
  
<span style="font-family:georgia,serif;">The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).</span>
+
Bregma levels were determined using a standard stereotaxic atlas of the rat brain (Paxinos and Watson, The rat brain in stereotaxic coordinates, Elsevier 2008) brain. For further details, see Stensrud et al. (Neuroscience 162:1055-71, 2009)
  
== <span style="font-size:smaller;"><span style="font-family:georgia,serif;"><span style="color: rgb(0, 0, 0); font-weight: bold;">Atlas coordinate values</span></span></span> ==
+
== <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="font-weight: bold">Contributing laboratories</span></span></span> ==
  
<span style="font-family:georgia,serif;">Positional coordinates representing distance from Bregma are indicated for each image in the repository. Values were estimated by matching by evaluation of multiple anatomical landmarks in comparison to a standard rat brain atlas (Paxinos and Watson, The Rat Brain in Stereotaxic Coordinates, Elsevier Academic Press, 2007).</span>
+
'''The Neuro- and Gliotransmitter Group''', Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Experimental work, immunohistochemistry. People: Mats Stensrud, Grazyna Babinska, Vidar Gundersen
  
== <span style="font-size:smaller;"><span style="font-family:georgia,serif;"><span style="color: rgb(0, 0, 0); font-weight: bold;">Data sharing</span></span></span> ==
+
'''Neural Systems Laboratory''' (http://www.nesys.uio.no), Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Image acquisition, atlas repository. People: Dmitri Darine, Gergely Csucs, Trygve B. Leergaard, Jan G. Bjaalie
  
<span style="font-family:georgia,serif;">Re-use of data from this repository is permitted provided reference is made to the following publication: Stensrud et al. J Comp Neurol, 521:3042-56, 2013. doi: 10.1002/cne.23331.</span>
+
== Funded by: ==
 +
The neuroinformatics components of this resource have been funded by the Human Brain Project through the European Union Seventh Framework Program (FP7/2007-2013) under grant agreement no. 604102 (HBP), and The Research Council of Norway (INCF National Node, project 218842).
  
== <span style="font-size:smaller;"><span style="font-family:georgia,serif;"><span style="color: rgb(0, 0, 0); font-weight: bold;">Access repository and Virtual Microscope</span></span></span> ==
+
== <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="font-weight: bold">Contact</span></span></span> ==
 
+
j.g.bjaalie@medisin.uio.no
Access [http://cmbn-navigator.uio.no/neurotransporter_atlas_vglut3/neurotransporter_atlas_vglut3_gui.html Navigator] to explore the data set.
 
 
 
== <span style="font-family:georgia,serif;"><span style="font-size:smaller;"><span style="font-weight: bold;">Contributing laboratories</span></span></span> ==
 
 
 
<span style="font-family:georgia,serif;">The Neuro- and Gliotransmitter Group - Vidar Gundersen</span>
 
 
 
<span style="font-family:georgia,serif;">Centre for Molecular Biology and Neuroscience &<br/>Institute of Basic Medical Sciences - Anatomy<br/>University of Oslo<br/>P.O. Box 1105 Blindern<br/>N - 0317 Oslo<br/>Norway<br/>[http://www.med.uio.no/imb/english/research/groups/synaptic-neurochemistry/gliotransmitters/index.html http://www.med.uio.no/imb/english/research/groups/synaptic-neurochemistry/gliotransmitters/index.html]<br/>[http://www.cmbn.no/ http://www.cmbn.no]</span>
 
 
 
 
 
 
 
<span style="font-family:georgia,serif;">Neural Systems and Graphics Computing Laboratory - Trygve B. Leergaard, Jan G. Bjaalie<br/>Centre for Molecular Biology and Neuroscience &<br/>Institute of Basic Medical Sciences - Anatomy<br/>University of Oslo<br/>P.O. Box 1105 Blindern<br/>N - 0317 Oslo<br/>Norway<br/>[http://www.nesys.uio.no/ http://www.nesys.uio.no]<br/>[http://www.rbwb.org/ http://www.rbwb.org]<br/>[http://www.cmbn.no/ http://www.cmbn.no]</span>
 
 
 
== <br/><span style="font-family:georgia,serif;"><span style="font-size:smaller;"><span style="font-weight: bold;">Credits</span></span></span> ==
 
 
 
=== <span style="font-family:georgia,serif;"><span style="font-size:smaller;">Immunohistochemistry</span></span> ===
 
 
 
<span style="font-family:georgia,serif;"><span style="font-size:small;">Grazyna Babinska<br/>Mats Julius Stensrud<br/>Vidar Gundersen</span></span>
 
 
 
=== <span style="font-family:georgia,serif;"><span style="font-size:smaller;">NT-atlas web-application development team:</span></span> ===
 
 
 
<span style="font-family:georgia,serif;"><span style="font-size:small;">Dmitri Darine<br/>Gergely Csucs<br/>Trygve B. Leergaard<br/>Jan G. Bjaalie</span></span>
 
 
 
== <span style="font-family:georgia,serif;"><span style="font-size:smaller;"><span style="font-weight: bold;">Requirements</span></span></span> ==
 
 
 
<span style="font-family:georgia,serif;">Web browser requirement:</span>
 
<ul style="color: rgb(0, 0, 0); font-family: Verdana, Arial, Helvetica, sans-serif;">
 
<li><span style="font-family:georgia,serif;">to view the Flash applications you need&nbsp;[http://www.adobe.com/go/getflashplayer Adobe Flash Player]&nbsp;installed on your computer</span></li>
 
</ul>
 

Revision as of 10:36, 20 September 2018

About

A: Punctuate dark-brown labeling representing VGLUT3-positive nerve endings in the pyramidal layer of the hippocampus. Scale bar, 50 µm.
B: VGLUT3 stained neuronal cell bodies (arrows) and processes (arrowheads) in the striatum. Scale bar, 50 µm.

Vesicular glutamate transporters (VGLUT1-3) carry glutamate into synaptic vesicles. The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain, visualized by immunohistochemistry using antibodies against VGLUT3.

Access image repository

The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link.

Re-use of data from this repository is allowed provided that reference is given to the following publication: Stensrud MJ, Chaudhry FA, Leergaard TB, Bjaalie JG, Gundersen V. Vesicular glutamate transporter-3 in the rodent brain: vesicular colocalization with vesicular γ-aminobutyric acid transporter. J Comp Neurol (2013) 521:3042–56. doi:10.1002/cne.23331

Image repository Download
Filmstrip viewer Tiffs

Experimental procedures in brief

The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).

Bregma levels were determined using a standard stereotaxic atlas of the rat brain (Paxinos and Watson, The rat brain in stereotaxic coordinates, Elsevier 2008) brain. For further details, see Stensrud et al. (Neuroscience 162:1055-71, 2009)

Contributing laboratories

The Neuro- and Gliotransmitter Group, Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Experimental work, immunohistochemistry. People: Mats Stensrud, Grazyna Babinska, Vidar Gundersen

Neural Systems Laboratory (http://www.nesys.uio.no), Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Image acquisition, atlas repository. People: Dmitri Darine, Gergely Csucs, Trygve B. Leergaard, Jan G. Bjaalie

Funded by:

The neuroinformatics components of this resource have been funded by the Human Brain Project through the European Union Seventh Framework Program (FP7/2007-2013) under grant agreement no. 604102 (HBP), and The Research Council of Norway (INCF National Node, project 218842).

Contact

j.g.bjaalie@medisin.uio.no