Neurotransporter Atlas: VGLUT3

From projects.navigator3
Revision as of 12:45, 21 June 2016 by Leergaar@uio.no (talk | contribs)

Jump to: navigation, search

About

Vesicular glutamate transporters (VGLUT1-3) carry glutamate into synaptic vesicles. The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain, visualized by immunohistochemistry using antibodies against VGLUT3.

|

Access image repository

The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link.

Re-use of data from this repository is allowed provided that reference is given to the following publication: Stensrud MJ, Chaudhry FA, Leergaard TB, Bjaalie JG, Gundersen V. Vesicular glutamate transporter-3 in the rodent brain: vesicular colocalization with vesicular γ-aminobutyric acid transporter. J Comp Neurol (2013) 521:3042–56. doi:10.1002/cne.23331

Image repository Download
Filmstrip viewer Tiffs

Experimental procedures in brief

The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).

Bregma levels were determined using a standard stereotaxic atlas of the rat brain (Paxinos and Watson, The rat brain in stereotaxic coordinates, Elsevier 2008) brain. For further details, see Stensrud et al. (Neuroscience 162:1055-71, 2009)

Contributing laboratories

The Neuro- and Gliotransmitter Group, Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Experimental work, immunohistochemistry. People: Mats Stensrud, Grazyna Babinska, Vidar Gundersen

Neural Systems Laboratory (http://www.nesys.uio.no), Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Image acquisition, atlas repository. People: Dmitri Darine, Gergely Csucs, Trygve B. Leergaard, Jan G. Bjaalie

Funded by:

The neuroinformatics components of this resource have been funded by the Human Brain Project through the European Union Seventh Framework Program (FP7/2007-2013) under grant agreement no. 604102 (HBP)

Contact

j.g.bjaalie@medisin.uio.no