Tg-Arc-Swe Atlas
About
The tg-ArcSwe atlas provides access to Aβx-40 immunolabeled histological section images from representative 12 month old tg-ArcSwe mice, showing the morphology and spatial distribution of Aβx-40 plaque deposits across the brain.
The Virtual Microscope tool allows users to navigate within and across sagittal section images.
Re-use of data from this repository is allowed provided that reference is given to the following publication: Lillehaug S, Syverstad GH, Nilsson LNG,Bjaalie JG, Leergaard TB, Torp R (2014): Brainwide distribution and variance of amyloid-beta deposits in tg-ArcSwe mice. Neurobiology og Aging, 35:556-564 (doi: 10.1016/j.neurobiolaging.2013.09.013).
Female tg-ArcSwe, 12 months (3x images, case F3)
Male tg-ArcSwe, 12 months (17 images, case M3)
Additional images
Female tg-ArcSwe, 12 months (2 images, case F1)
Relatively loose plaque morphology
Male tg-ArcSwe, 12 months (2 images, case M4)
Small, dense plaque deposits
For details, please cf. Lillehaug, Syverstad et al., (Neurobiology of Aging, 2014, 35:556-564).
Animals: mice carrying a human APP cDNA with the Arctic (p. E693G) and Swedish (p. KM670/671NL) mutations
Immunohistochemistry:
Sagittal sections from right hemisphere were cryosectioned at 25 µm.
Amyloid deposits were identified using primary antibodies Aβx-40, visualized using streptavidin-biotinylated horseradish peroxidase comples and diaminobenzidine tetrahydrochloride
Digital images were created using a slidescanner (Mirax Scan, Carl Zeiss Microimaging, Jena, Germany)
Credits
Animal breeding: Reidun Torp, Lars N. G. Nilsson
Immunohistochemistry: Gry H. Syverstad, Reidun Torp, Grazyna Babinska
tg-ArcSwe atlas development team: Sveinung Lillehaug, Dmitri Darine, Gergely Scucs, Jan G. Bjaalie, Trygve B. Leergaard
Sveinung Lillehaug, Jan G. Bjaalie, Trygve B. Leergaard Neural Systems and Graphics Computing Laboratory Institute of Basic Medical Sciences - Anatomy University of Oslo P.O. Box 1105 Blindern N - 0317 Oslo Norway http://www.nesys.uio.no http://www.rbwb.org
Gry Helen Enger Syverstad, Reidun Torp Laboratory of Molecular Neuroscience Institute of Basic Medical Sciences - Anatomy University of Oslo P.O. Box 1105 Blindern N - 0317 Oslo Norway
Lars N.G. Nilsson Department of Pharmacology, Institute of Clinical Medicine, University of Oslo & Oslo University Hospital P.O. Box 1057 Blindern N - 0316 Oslo Norway
