Difference between revisions of "Unresolved issues"
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| − | + | There are several unresolved issues present when it comes to the experiment part of the project. | |
| + | |||
| + | The following issues are: | ||
| + | |||
| + | == Zimmer & Peacock pH sensor == | ||
| + | == Keeping the cells alive inside the chip == | ||
| + | In order to achieve useful results | ||
| + | |||
| + | '''Possible solutions:''' | ||
| + | |||
| + | == Controlling the CO2 levels == | ||
| + | Environment | ||
| + | |||
| + | '''Possible solutions:''' | ||
| + | |||
| + | == Air bubbles == | ||
| + | When filling chambers with liquid, the bubbles can result in the cells dying prematurely and/or inhibiting the flow. | ||
| + | |||
| + | '''Possible solutions to air bubbles:''' | ||
| + | * The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber. | ||
| + | * Adding soft surfacant to make sure the bubbles detach from the surface. | ||
| + | * Being highly aware of pressure levels when adding liquids to the chambers. Even the smallest changes in pressure can result in air bubbles. | ||
| + | * [https://darwin-microfluidics.com/products/microfluidic-bubble-trap Bubble Trap for Microfluidics Kit] provided by [https://www.elveflow.com/ Elveflow] which gets rid of the air bubbles before they enter the chip. | ||
| + | *[https://www.elveflow.com/microfluidic-reviews/general-microfluidics/air-bubbles-and-microfluidics/#:~:text=Bubbles%20dissolution%3A%20For%20those%20that,to%20dissolve%20into%20the%20liquid. Air bubbles and microfluidics: Tips and tricks to remove them] | ||
| + | *[https://www.elveflow.com/microfluidic-reviews/microfluidics-for-cell-biology/methods-and-techniques-perfusion-cell-culture/ Methods & techniques for perfusion cell culture] | ||
Latest revision as of 22:03, 9 August 2022
There are several unresolved issues present when it comes to the experiment part of the project.
The following issues are:
Contents
Zimmer & Peacock pH sensor
Keeping the cells alive inside the chip
In order to achieve useful results
Possible solutions:
Controlling the CO2 levels
Environment
Possible solutions:
Air bubbles
When filling chambers with liquid, the bubbles can result in the cells dying prematurely and/or inhibiting the flow.
Possible solutions to air bubbles:
- The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber.
- Adding soft surfacant to make sure the bubbles detach from the surface.
- Being highly aware of pressure levels when adding liquids to the chambers. Even the smallest changes in pressure can result in air bubbles.
- Bubble Trap for Microfluidics Kit provided by Elveflow which gets rid of the air bubbles before they enter the chip.
- Air bubbles and microfluidics: Tips and tricks to remove them
- Methods & techniques for perfusion cell culture
