Cell lab 431

Usage

The purpose of this SOP is to describe routines for the use of cell culture lab, room 431 on the 4th floor of west wing in the Physics building.

All users of the cell lab must have direct training from the room responsible person before room is used. New user must sign the completed training form.

The room must be booked in BookitLab. UiO has a BookitLab user guide.

List of maintenance procedures

Preparation of solutions

• DMEM
• PBS

Lab maintenance

• Autoclaving
• Vacusafe
• Incubator
• Cleaning
• Waste disposal
• List of standard items

Cell culturing

Cell lines

• Hela
• MDCK
• RPE
• BV-2
• Muscle cells

Other

• Collagen for 3D or coating surfaces

Protocols

• Micropatterning on hydrogels