Difference between revisions of "05.05.23"
(Tag: Visual edit) |
(Tag: Visual edit) |
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* Felix | * Felix | ||
| − | ==== Results (rotations per 10sec): ==== | + | ====Results (rotations per 10sec): ==== |
Each row for a clip is a different cell. | Each row for a clip is a different cell. | ||
{| class="wikitable" | {| class="wikitable" | ||
| Line 26: | Line 26: | ||
|3 | |3 | ||
|2.125 | |2.125 | ||
| − | | | + | |1.875 |
| − | | | + | |1.5 |
| − | |0 | + | |0.5 |
| − | | | + | |1.125 |
| − | |0 | + | | -0.25 |
| − | |0 | + | | -0.375 |
| − | |0 | + | | -0.75 |
| − | | | + | | -1 |
| − | |0 | + | | -0.75 |
|- | |- | ||
|0.375 | |0.375 | ||
| Line 40: | Line 40: | ||
|1.125 | |1.125 | ||
|2.215 | |2.215 | ||
| − | | | + | |1.75 |
| − | | | + | |1.125 |
| − | | | + | |1 |
| − | |0 | + | |0.875 |
| − | |0 | + | | -0.375 |
| − | |0 | + | | -0.25 |
| − | | | + | | -1.125 |
| − | | | + | | -1 |
| − | |0 | + | | -0.75 |
|- | |- | ||
|0.375 | |0.375 | ||
| Line 54: | Line 54: | ||
|2.625 | |2.625 | ||
|1.625 | |1.625 | ||
| − | | | + | |1.875 |
| − | | | + | |1.625 |
| − | | | + | |1.125 |
| − | |0 | + | |0.5 |
| − | |0 | + | | -0.25 |
| − | |0 | + | | -0.375 |
| − | |0 | + | | -0.625 |
| − | | | + | | -1 |
| − | |0 | + | | -0.875 |
|} | |} | ||
| Line 70: | Line 70: | ||
- We were a bit inefficient in starting the experiment after preparing the sample, which may also have affected the data. | - We were a bit inefficient in starting the experiment after preparing the sample, which may also have affected the data. | ||
| + | |||
| + | - Some cells in the 200-500 khz range were *a lot* faster than all their neighbours, so they were ignored as outliers. | ||
| + | |||
| + | - Data plotted below. In right figure we get a similar average spectra from all our KO data, no apparent significant change from [[14.04.23]]. | ||
| + | |||
| + | - One difference with these data and previous KO experiments is that the peak is at 200kHz (just barely higher than 100kHz). This still does not significantly change the average KO spectra (right fig), but it could be a consequence of the delayed experiment (physiological decay of cells / lysis?) or just a statistical deviation (much variation at the peak). | ||
| + | |||
| + | [[File:BV2 KO Rotation (2).png|left|thumb|720x720px|Data plotted with previous KO data]] | ||
| + | [[File:BV2 KO WT (1).png|thumb|720x720px|The average KO rotation spectra is not significantly changed from the results seen from [[14.04.23|14.04.23.]]]] | ||
Latest revision as of 11:59, 7 May 2023
Present:
- Nigar
- Lasse
- Morten
- Felix
Results (rotations per 10sec):
Each row for a clip is a different cell.
| 10kHz | 100kHz | 200kHz | 300kHz | 400kHz | 500kHz | 1MHz | 5Mhz | 15MHz | 20MHz | 30MHz | 40MHz | 50MHz |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.5 | 3.125 | 3 | 2.125 | 1.875 | 1.5 | 0.5 | 1.125 | -0.25 | -0.375 | -0.75 | -1 | -0.75 |
| 0.375 | 1.25 | 1.125 | 2.215 | 1.75 | 1.125 | 1 | 0.875 | -0.375 | -0.25 | -1.125 | -1 | -0.75 |
| 0.375 | 2.125 | 2.625 | 1.625 | 1.875 | 1.625 | 1.125 | 0.5 | -0.25 | -0.375 | -0.625 | -1 | -0.875 |
Observations:
- We did a rotation on KO cells. For some reason, we identified a lot of cell lysate, so somethng mght have gone wrong during passaging, or with the EDTA (maybe it is too old). We acquired data nevertheless, and uploaded on the shared Google Drive.
- We were a bit inefficient in starting the experiment after preparing the sample, which may also have affected the data.
- Some cells in the 200-500 khz range were *a lot* faster than all their neighbours, so they were ignored as outliers.
- Data plotted below. In right figure we get a similar average spectra from all our KO data, no apparent significant change from 14.04.23.
- One difference with these data and previous KO experiments is that the peak is at 200kHz (just barely higher than 100kHz). This still does not significantly change the average KO spectra (right fig), but it could be a consequence of the delayed experiment (physiological decay of cells / lysis?) or just a statistical deviation (much variation at the peak).
.png)
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