Difference between revisions of "Diluted PBS"
(Created page with "== Preparing diluted PBS == '''Instruction:''' 1<sup>st</sup>: Transfer 50 ml PBS stock solution into the empty 500 ml borosilicate glass bottle (work inside the bio LAF ben...") |
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| + | == Required Items: == | ||
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| + | * 25 ml Serological Pipette | ||
| + | * 500 mL volume metric flask | ||
| + | * empty 500 ml borosilicate glass bottle | ||
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| + | Stock solution: (PBS - PHOSPHATE BUFFERED SALINE 10× concentrate - P5493) from sigma / Merck, Type II water | ||
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== Preparing diluted PBS == | == Preparing diluted PBS == | ||
'''Instruction:''' | '''Instruction:''' | ||
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3<sup>rd</sup>: Autoclave the diluted PBS solution before using. | 3<sup>rd</sup>: Autoclave the diluted PBS solution before using. | ||
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'''Work Steps''' | '''Work Steps''' | ||
Revision as of 11:41, 17 November 2023
Required Items:
- 25 ml Serological Pipette
- 500 mL volume metric flask
- empty 500 ml borosilicate glass bottle
Stock solution: (PBS - PHOSPHATE BUFFERED SALINE 10× concentrate - P5493) from sigma / Merck, Type II water
Preparing diluted PBS
Instruction:
1st: Transfer 50 ml PBS stock solution into the empty 500 ml borosilicate glass bottle (work inside the bio LAF bench to avoid polluting the stock PBS solution)
2nd: Transfer 450 ml type II water into the same above 500 ml borosilicate glass bottle. (work outside the LAF bench, on the lab bench)
3rd: Autoclave the diluted PBS solution before using.
Work Steps
Step 1: Use 75% antibac ethanol alcohol to spray the pack surface of 25 ml Serological Pipette, the surface of the 500 ml borosilicate glass bottle and the bottle of stock solution.
Step 2: right after spray each item and place them inside the bio LAF hood.
Step 3: Transfer 50 ml PBS stock solution into the empty 500 ml borosilicate glass bottle and close the cap, then take out the bottle from LAF.
Step 4: transfer 450 ml type II water with 500 mL volume metric flask into the 500 ml borosilicate glass bottle. (Work outside the LAF fume hood – just on lab bench).
Step 5: Autoclave the diluted PBS solution.
