Difference between revisions of "Diluted PBS"
| Line 1: | Line 1: | ||
== Required Items: == | == Required Items: == | ||
| − | * 25 | + | * 25 mL Serological Pipette |
* 500 mL volume metric flask | * 500 mL volume metric flask | ||
| − | * 500 | + | * 500 mL borosilicate glass bottle |
| − | * | + | * PBS - PHOSPHATE BUFFERED SALINE 10× concentrate - P5493 from sigma / Merck |
* Type II water | * Type II water | ||
Revision as of 11:43, 17 November 2023
Required Items:
- 25 mL Serological Pipette
- 500 mL volume metric flask
- 500 mL borosilicate glass bottle
- PBS - PHOSPHATE BUFFERED SALINE 10× concentrate - P5493 from sigma / Merck
- Type II water
Preparing diluted PBS
Instruction:
1st: Transfer 50 ml PBS stock solution into the empty 500 ml borosilicate glass bottle (work inside the bio LAF bench to avoid polluting the stock PBS solution)
2nd: Transfer 450 ml type II water into the same above 500 ml borosilicate glass bottle. (work outside the LAF bench, on the lab bench)
3rd: Autoclave the diluted PBS solution before using.
Work Steps
Step 1: Use 75% antibac ethanol alcohol to spray the pack surface of 25 ml Serological Pipette, the surface of the 500 ml borosilicate glass bottle and the bottle of stock solution.
Step 2: right after spray each item and place them inside the bio LAF hood.
Step 3: Transfer 50 ml PBS stock solution into the empty 500 ml borosilicate glass bottle and close the cap, then take out the bottle from LAF.
Step 4: transfer 450 ml type II water with 500 mL volume metric flask into the 500 ml borosilicate glass bottle. (Work outside the LAF fume hood – just on lab bench).
Step 5: Autoclave the diluted PBS solution.
