Difference between revisions of "Maintenance"

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# List of things to have all the time in 431 #
 
* Always at least one full bottle of autoclaved water
 
* Always at least one full spray flask of 75% alcohol
 
* Always one half full bottle of DMEM supplied with extras
 
* Yellow waste container no more than 3/4 filled
 
  
 
# List of procedures #
 
 
## Autoclaved water ##
 
Must be done 4-5 hours before use of water (for cooling down)
 
* Get 5l bottles of water from chemistry (check quality resistivity > 1MOhm)
 
* Fill 1l glass bottle with water
 
* put cap on, untighten cap
 
* put autoclave scotch on
 
* put in autoclave and follow procedure on autoclave
 
 
## Dulbeccos Modified Eagle Media, DMEM adding extras##
 
* Inside sterile hood with sterile procedures
 
* Open new bottle of DMEM
 
* Add 50 ml FBS
 
* Add 5 ml Penicilin Streptomycin
 

Latest revision as of 15:21, 17 November 2023