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| − | ### List of things to have all the time in 431 ###
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| − | * Always at least one full bottle of autoclaved water
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| − | * Always at least one full spray flask of 75% alcohol
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| − | * Always one half full bottle of DMEM supplied with extras
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| − | * Yellow waste container no more than 3/4 filled
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| − | ## List of procedures ##
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| − | ## Autoclaved water ##
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| − | Must be done 4-5 hours before use of water (for cooling down)
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| − | * Get 5l bottles of water from chemistry (check quality resistivity > 1MOhm)
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| − | * Fill 1l glass bottle with water
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| − | * put cap on, untighten cap
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| − | * put autoclave scotch on
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| − | * put in autoclave and follow procedure on autoclave
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| − | ## Dulbeccos Modified Eagle Media, DMEM adding extras##
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| − | * Inside sterile hood with sterile procedures
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| − | * Open new bottle of DMEM
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| − | * Add 50 ml FBS
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| − | * Add 5 ml Penicilin Streptomycin
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