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| − | = List of things to have all the time in 431 =
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| − | * Always at least one full bottle of autoclaved water
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| − | * Always at least one full spray flask of 75% alcohol
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| − | * Always one half full bottle of DMEM supplied with extras
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| − | * Yellow waste container no more than 3/4 filled
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| − | * Check that sticky mat sticks
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| − | * Empty Vacusafe when it is more than half full
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| − | * Once a week, refill sterile water in incubator. Need: 1-2 l.
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| − | * When the diluted PBS bottle is less than half full prepare another
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| − | * Check the list of things to buy (on the wall in the fore-room)
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| − | * Check the CO2 level in bottles. Reorder bottle if necessary.
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| − | * Always at least 20 sterile pasteur pipettes in hood, if not: autoclave new
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| − |
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| − | = List of procedures =
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| − | == Autoclaved water ==
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| − | Must be done 4-5 hours before use of water (for cooling down)
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| − | * Get 5l bottles of water from chemistry (check quality resistivity > 1MOhm)
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| − | * Fill 1l glass bottle with water
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| − | * put cap on, untighten cap
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| − | * put autoclave scotch on
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| − | * put in autoclave and follow procedure on autoclave
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| − | == Dulbeccos Modified Eagle Media, DMEM adding extras ==
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| − | * Inside sterile hood with sterile procedures
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| − | * Open new bottle of DMEM
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| − | * Add 50 ml FBS
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| − | * Add 5 ml Penicilin Streptomycin
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| − | == Vacusafe ==
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| − | * Add chlorine?
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| − | * Pour into yellow waste container ?
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| − | == Refilling water in incubator ==
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| − | * Have 1-2 l sterile water ready and spray top around lid
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| − | * Put on gloves and spray gloves
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| − | * Open incubator
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| − | * withdraw water pan half way
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| − | * pour water in to half full
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| − |
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| − | == Preparing diluted PBS ==
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| − | * zdf
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| − | == Autoclaving pasteur pipettes ==
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| − | * stock in cupboard in 431
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| − | * put them in a 1l beaker
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| − | * ...
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