Difference between revisions of "Cell culturing"

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== Collagen for 3D or coating surfaces ==
 
== Collagen for 3D or coating surfaces ==
Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this
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Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating surfaces, as it can form thin layers for culturing cells.
fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell
 
environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating
 
surfaces, as it can form thin layers for culturing cells.
 
  
Feb 2021 we bought Type 1 Collagen from Ibidi (through Inter Instrument https://interinst.no/).
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[https://ibidi.com/cell-culture-microscopy/107-collagen-type-i-rat-tail.html#/110-concentration_volume-5_mg_ml_4_x_5_ml Type 1 Collagen from Ibidi],  '''[https://ibidi.com/img/cms/products/cells_reagents/R_5020X_CollagenI/IN_5020X_CollagenI_05mg.pdf Protocols for use.]''' (bought through Inter Instrument https://interinst.no/. The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA Feb 2021).
The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA
 
 
 
[[Protocols for Type 1 Collagen IB 50202]]
 

Revision as of 14:44, 19 March 2021

Hela cells

Muscle cells

Collagen for 3D or coating surfaces

Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating surfaces, as it can form thin layers for culturing cells.

Type 1 Collagen from Ibidi, Protocols for use. (bought through Inter Instrument https://interinst.no/. The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA Feb 2021).