Difference between revisions of "Cell culturing"
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== Muscle cells == | == Muscle cells == | ||
+ | In the Bioactuator project, we use C2C12 myoblasts. For more information about C2C12 myoblasts click on the following link: https://en.wikipedia.org/wiki/C2C12#:~:text=C2C12%20is%20an%20immortalized%20mouse%20myoblast%20cell%20line.&text=C2C12%20cells%20are%20used%20to,to%20explore%20mechanistic%20biochemical%20pathways. | ||
== Collagen for 3D or coating surfaces == | == Collagen for 3D or coating surfaces == |
Revision as of 08:49, 13 April 2021
Hela cells
Muscle cells
In the Bioactuator project, we use C2C12 myoblasts. For more information about C2C12 myoblasts click on the following link: https://en.wikipedia.org/wiki/C2C12#:~:text=C2C12%20is%20an%20immortalized%20mouse%20myoblast%20cell%20line.&text=C2C12%20cells%20are%20used%20to,to%20explore%20mechanistic%20biochemical%20pathways.
Collagen for 3D or coating surfaces
Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating surfaces, as it can form thin layers for culturing cells.
Type 1 Collagen from Ibidi, Protocols for use. (bought through Inter Instrument https://interinst.no/. The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA Feb 2021).