Difference between revisions of "Maintenance"

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(Created page with "# List of things to have all the time in 431 # * Always at least one full bottle of autoclaved water * Always at least one full spray flask of 75% alcohol * Always one half fu...")
 
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# List of things to have all the time in 431 #
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### List of things to have all the time in 431 ###
 
* Always at least one full bottle of autoclaved water
 
* Always at least one full bottle of autoclaved water
 
* Always at least one full spray flask of 75% alcohol
 
* Always at least one full spray flask of 75% alcohol
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# List of procedures #
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## List of procedures ##
  
 
## Autoclaved water ##
 
## Autoclaved water ##

Revision as of 11:25, 20 April 2021

      1. List of things to have all the time in 431 ###
  • Always at least one full bottle of autoclaved water
  • Always at least one full spray flask of 75% alcohol
  • Always one half full bottle of DMEM supplied with extras
  • Yellow waste container no more than 3/4 filled


    1. List of procedures ##
    1. Autoclaved water ##

Must be done 4-5 hours before use of water (for cooling down)

  • Get 5l bottles of water from chemistry (check quality resistivity > 1MOhm)
  • Fill 1l glass bottle with water
  • put cap on, untighten cap
  • put autoclave scotch on
  • put in autoclave and follow procedure on autoclave
    1. Dulbeccos Modified Eagle Media, DMEM adding extras##
  • Inside sterile hood with sterile procedures
  • Open new bottle of DMEM
  • Add 50 ml FBS
  • Add 5 ml Penicilin Streptomycin