Difference between revisions of "Maintenance"
From mn.fysikk.laglivlab
Dagkd@uio.no (talk | contribs) |
Dagkd@uio.no (talk | contribs) |
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| − | + | = List of things to have all the time in 431 = | |
* Always at least one full bottle of autoclaved water | * Always at least one full bottle of autoclaved water | ||
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| − | + | == List of procedures == | |
| − | + | == Autoclaved water == | |
Must be done 4-5 hours before use of water (for cooling down) | Must be done 4-5 hours before use of water (for cooling down) | ||
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* put in autoclave and follow procedure on autoclave | * put in autoclave and follow procedure on autoclave | ||
| − | + | == Dulbeccos Modified Eagle Media, DMEM adding extras == | |
* Inside sterile hood with sterile procedures | * Inside sterile hood with sterile procedures | ||
Revision as of 11:27, 20 April 2021
Contents
List of things to have all the time in 431
- Always at least one full bottle of autoclaved water
- Always at least one full spray flask of 75% alcohol
- Always one half full bottle of DMEM supplied with extras
- Yellow waste container no more than 3/4 filled
List of procedures
Autoclaved water
Must be done 4-5 hours before use of water (for cooling down)
- Get 5l bottles of water from chemistry (check quality resistivity > 1MOhm)
- Fill 1l glass bottle with water
- put cap on, untighten cap
- put autoclave scotch on
- put in autoclave and follow procedure on autoclave
Dulbeccos Modified Eagle Media, DMEM adding extras
- Inside sterile hood with sterile procedures
- Open new bottle of DMEM
- Add 50 ml FBS
- Add 5 ml Penicilin Streptomycin
