Difference between revisions of "Maintenance"
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Dagkd@uio.no (talk | contribs) |
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* Check the list of things to buy (on the wall in the fore-room) | * Check the list of things to buy (on the wall in the fore-room) | ||
* Check the CO2 level in bottles. Reorder bottle if necessary. | * Check the CO2 level in bottles. Reorder bottle if necessary. | ||
+ | * Always at least 20 sterile pasteur pipettes in hood, if not: autoclave new | ||
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== Preparing diluted PBS == | == Preparing diluted PBS == | ||
* zdf | * zdf | ||
+ | |||
+ | == Autoclaving pasteur pipettes == | ||
+ | * stock in cupboard in 431 | ||
+ | * put them in a 1l beaker | ||
+ | * ... |
Revision as of 12:19, 20 April 2021
Contents
List of things to have all the time in 431
- Always at least one full bottle of autoclaved water
- Always at least one full spray flask of 75% alcohol
- Always one half full bottle of DMEM supplied with extras
- Yellow waste container no more than 3/4 filled
- Check that sticky mat sticks
- Empty Vacusafe when it is more than half full
- Once a week, refill sterile water in incubator. Need: 1-2 l.
- When the diluted PBS bottle is less than half full prepare another
- Check the list of things to buy (on the wall in the fore-room)
- Check the CO2 level in bottles. Reorder bottle if necessary.
- Always at least 20 sterile pasteur pipettes in hood, if not: autoclave new
List of procedures
Autoclaved water
Must be done 4-5 hours before use of water (for cooling down)
- Get 5l bottles of water from chemistry (check quality resistivity > 1MOhm)
- Fill 1l glass bottle with water
- put cap on, untighten cap
- put autoclave scotch on
- put in autoclave and follow procedure on autoclave
Dulbeccos Modified Eagle Media, DMEM adding extras
- Inside sterile hood with sterile procedures
- Open new bottle of DMEM
- Add 50 ml FBS
- Add 5 ml Penicilin Streptomycin
Vacusafe
- Add chlorine?
- Pour into yellow waste container ?
Refilling water in incubator
- Have 1-2 l sterile water ready and spray top around lid
- Put on gloves and spray gloves
- Open incubator
- withdraw water pan half way
- pour water in to half full
Preparing diluted PBS
- zdf
Autoclaving pasteur pipettes
- stock in cupboard in 431
- put them in a 1l beaker
- ...