Revision as of 13:07, 20 April 2021

List of things to have all the time in 431

• Sterile water: Always at least one full bottle of autoclaved water
• Always at least one full spray flask of 75% alcohol
• Always one half full bottle of DMEM supplied with extras
• Yellow waste container no more than 3/4 filled
• Check that sticky mat sticks
• Empty Vacusafe when it is more than half full
• Once a week, refill sterile water in incubator. Need: 1-2 l.
• When the diluted PBS bottle is less than half full prepare another
• Check the list of things to buy (on the wall in the fore-room)
• Check the CO2 level in bottles. Reorder bottle if necessary.
• Always at least 20 sterile pasteur pipettes in hood, if not: autoclave new
• Every 3 weeks: wash coats

List of procedures

Autoclaved water

Must be done 4-5 hours before use of water (for cooling down)

• Get 5l bottles of water from chemistry (check quality resistivity > 1MOhm)
• Fill 1l glass bottle with water
• put cap on, untighten cap
• put autoclave scotch on
• put in autoclave and follow procedure on autoclave

Dulbeccos Modified Eagle Media, DMEM adding extras

• Inside sterile hood with sterile procedures
• Open new bottle of DMEM
• Add 50 ml FBS
• Add 5 ml Penicilin Streptomycin

Vacusafe

• Add chlorine?
• Pour into yellow waste container ?

Refilling water in incubator

• Have 1-2 l sterile water ready and spray top around lid
• Put on gloves and spray gloves
• Open incubator
• withdraw water pan half way
• pour water in to half full

Preparing diluted PBS

• zdf

Autoclaving pasteur pipettes

• stock in cupboard in 431
• put them in a 1l beaker
• ...